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https://hdl.handle.net/123456789/1081| Τύπος: | Άρθρο σε επιστημονικό περιοδικό |
| Τίτλος: | Liquid biopsy genotyping by a simple lateral flow strip assay with visual detection |
| Συγγραφέας: | [EL] Καλλιγοσφύρη, Παναγιώτα[EN] Kalligosfyri, Panagiota [EL] Νίκου, Σοφία[EN] Nikou, Sofia [EL] Μπράβου, Βασιλική[EN] Bravou, Vasiliki [EL] Καλογιάννη, Δέσποινα[EN] Kalogianni, Despoina |
| Ημερομηνία: | 12/04/2021 |
| Περίληψη: | Liquid biopsy, as a minimally invasive method that allows real-time monitoring of the tumor genome, represents a competing approach for cancer diagnosis, prognosis and therapy decision making. Liquid biopsy in cancer patients mainly includes analysis of circulating tumor cells (CTC) and cell-free circulating tumor DNA (ctDNA). ctDNA is the tumor-derived fraction of the cell-free DNA present in the blood. ctDNA is detected based on cancer-specific genomic aberrations (mainly mutations) and represents a challenging analyte due to high fragmentation and low concentration. Several methodologies have been developed for ctDNA analysis in cancer patients but many of these technologies are too time-intensive, complicated and expensive for implementation in diagnostic testing. Herein, we developed a novel lateral flow strip assay for mutational analysis of ctDNA in blood samples and visual detection that is based on gold nanoparticles as reporters. As a model, common single-point mutations of the KRAS gene, related to colorectal cancer (CRC), have been selected for method development. The proposed DNA biosensor has been successfully applied for the detection of three KRAS mutations (KRAS G12D/A/V), along with the wild-type KRAS gene in synthetic DNA targets, cancer cell lines and cfDNA from blood samples of healthy individuals and CRC patients. The main advantages of the proposed lateral flow assay are simplicity, rapid analysis time (~10 min) and visual detection without the requirement of special instrumentation. The assay is also cost-effective with high detectability, specificity and reproducibility and has the potential to be used as a portable and universal device. In conclusion, the proposed assay offers a rapid diagnostic strip test for visual genotyping, as an alternative approach for liquid biopsy applications. |
| Γλώσσα: | Αγγλικά |
| Σελίδες: | 10 |
| DOI: | 10.1016/j.aca.2021.338470 |
| ISSN: | 0003-2670 |
| Θεματική κατηγορία: | [EL] Άλλες φυσικές επιστήμες[EN] Other natural sciences |
| Λέξεις-κλειδιά: | Biosensors; Liquid Biopsy; gold nanoparticles; lateral flow assay; KRAS mutations; Dipstick; KRAS; Cell-free DNA; circulating tumor DNA |
| Κάτοχος πνευματικών δικαιωμάτων: | © 2021 Elsevier B.V. All rights reserved. |
| Ηλεκτρονική διεύθυνση του τεκμηρίου στον εκδότη: | https://www.sciencedirect.com/science/article/pii/S0003267021002968 |
| Ηλεκτρονική διεύθυνση περιοδικού: | https://www.sciencedirect.com/journal/analytica-chimica-acta |
| Τίτλος πηγής δημοσίευσης: | Analytica Chimica Acta |
| Τεύχος: | 1163 |
| Σελίδες τεκμηρίου (στην πηγή): | Article no 338470 |
| Σημειώσεις: | This research is co-financed by Greece and the European Union (European Social Fund - ESF) through the Operational Programme « Human Resources Development, Education and Lifelong Learning 2014e2020» in the context of the project “Gold nanoparticle-based biosensor for rapid liquid biopsy applications” (MIS 5047147). |
| Εμφανίζεται στις συλλογές: | Ερευνητικές ομάδες |
Αρχεία σε αυτό το τεκμήριο:
| Αρχείο | Περιγραφή | Σελίδες | Μέγεθος | Μορφότυπος | Έκδοση | Άδεια | |
|---|---|---|---|---|---|---|---|
| 21ACA KRAS.pdf Restricted Access | 1.27 MB | Adobe PDF | - |  | Δείτε/ανοίξτε |