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https://hdl.handle.net/123456789/1083| Τύπος: | Ανακοίνωση σε συνέδριο |
| Τίτλος: | Gold nanoparticle-based biosensor for rapid liquid biopsy applications |
| Συγγραφέας: | [EL] Καλλιγοσφύρη, Παναγιώτα[EN] Kalligosfyri, Panagiota [EL] Νίκου, Σοφία[EN] Nikou, Sofia [EL] Μπράβου, Βασιλική[EN] Bravou, Vasiliki [EL] Καλογιάννη, Δέσποινα[EN] Kalogianni, Despoina |
| Ημερομηνία: | 09/07/2020 |
| Περίληψη: | Liquid biopsy offers a non-invasive, easy, quick and convenient alternative approach for early diagnosis of cancer and real-time monitoring of tumor progression. Liquid biopsies assess various biomarkers mainly represented by blood circulating tumor cells (CTC), circulating tumor DNA (ctDNA), circulating microRNAs and exosomes that are secreted by the tumors. Using body fluid samples, liquid biopsies may be the ideal approach for the detection of these biomarkers. The presence of circulating tumor DNA (ctDNA) or cell-free DNA (cfDNA), derived from tumors, in blood circulation is of major importance for early cancer detection, monitor tumor progress or diagnose the presence of activating mutations to guide treatment. The ability to detect ctDNA can serve as a liquid biopsy clinical application. In this project, a new gold nanoparticles-based DNA biosensor was developed for the detection of the most significant/common mutations in KRAS gene related to colorectal cancer in cell-free tumor DNA. Three major mutations and the normal allele of KRAS gene were detected by the biosensor. The biosensor was firstly optimized using synthetic DNA targets and was then applied to specific cell lines and blood serum samples. DNA and cell-free DNA were isolated from the samples, amplified, subjected to allele-discrimination reaction and analysed with the biosensor. This biosensor offers simplicity, as no expensive instrumentation is required, rapid analysis that is completed within 10 min, high detectability, specificity and reproducibility, while the detection is accomplished by naked eye. |
| Γλώσσα: | Αγγλικά |
| Τόπος δημοσίευσης: | Θεσσαλονίκη, Ελλάδα |
| Σελίδες: | 1 |
| Θεματική κατηγορία: | [EL] Άλλες φυσικές επιστήμες[EN] Other natural sciences |
| Λέξεις-κλειδιά: | Biosensors; Liquid Biopsy; Lateral flow assay; Gold nanoparticles |
| Κάτοχος πνευματικών δικαιωμάτων: | © The Author(s) 2020 |
| Ηλεκτρονική διεύθυνση του τεκμηρίου στον εκδότη: | https://www.nanotexnology.com/2020/index.php/nn20-proceedings |
| Τίτλος πηγής δημοσίευσης: | 17th International Conference on Nanosciences & Nanotechnologies Abstracts Book |
| Σελίδες τεκμηρίου (στην πηγή): | 65 |
| Όνομα εκδήλωσης: | 17th International Conference on Nanosciences & Nanotechnologies |
| Τοποθεσία εκδήλωσης: | Thessaloniki, Greece |
| Ημ/νία έναρξης εκδήλωσης: | 07/07/2020 |
| Ημ/νία λήξης εκδήλωσης: | 10/07/2020 |
| Σημειώσεις: | “This research is co-financed by Greece and the European Union (European Social Fund- ESF) through the Operational Programme «Human Resources Development, Education and Lifelong Learning 2014-2020» in the context of the project “Gold nanoparticle-based biosensor for rapid liquid biopsy applications” (MIS 5047147).” |
| Εμφανίζεται στις συλλογές: | Ερευνητικές ομάδες |
Αρχεία σε αυτό το τεκμήριο:
| Αρχείο | Περιγραφή | Σελίδες | Μέγεθος | Μορφότυπος | Έκδοση | Άδεια | |
|---|---|---|---|---|---|---|---|
| NN20_Abstract_book page 65.pdf | 3.55 MB | Adobe PDF | - |  | Δείτε/ανοίξτε |