1. Αποθετήριο Παραδοτέων Υποτρόφων Δράσεων ΕΣΠΑ Τριτοβάθμιας Εκπαίδευσης 2017-2023
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3. Ερευνητικές ομάδες

Τύπος:	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Anticancer activity of biogenic selenium nanoparticles: apoptotic and immunogenic cell death markers in colon cancer cells
Συγγραφέας:	[EL] Σπυριδοπούλου, Αικατερίνη[EN] Spyridopoulou, Katerina [EL] Αϊνδελής, Γεώργιος[EN] Aindelis, Georgios [EL] Παππά, Αγλαΐα[EN] Pappa, Aglaia [EL] Χλίχλια, Αικατερίνη[EN] Chlichlia, Aikaterini
Ημερομηνία:	24/10/2021
Περίληψη:	Colorectal cancer is a health problem with high mortality rates and prevalence. Thus, innovative treatment approaches need to be developed. Biogenic nanoparticles are nanomaterials that can be synthesised in biological systems and, compared to chemically synthesised nanoparticles, have better bioavailability while being more cost-effective, eco-friendlier, and less toxic. In our previous studies, the probiotic strain Lactobacillus casei ATCC 393 was used to synthesise selenium nanoparticles (SeNps), which were shown to inhibit colon cancer cell growth in vitro and in vivo. Herein, we have further investigated SeNps’ pro-apoptotic activity and their ability to induce immunogenic cell death (ICD) in colon cancer cells. The SeNps’ effect on Caco-2 cells growth was examined along with their potential to induce caspase activation. Moreover, the expression of typical pro-apoptotic and ICD markers were examined in SeNps-treated HT29 and CT26 cells by flow cytometry, Western blot, ELISA and fluorescence microscopy. Elevated caspase-3 activation and surface phosphatyldoserine, that subsided upon co-incubation with a pan-caspase inhibitor, were detected in SeNps-treated cells. Furthermore, nanoparticles induced modulation of the expression of various apoptosis-related proteins. We also report the detection of biomarkers involved in ICD, namely the translocation of calreticulin and ERp57, the release of HMGB1 and ATP, and the secretion of pro-inflammatory cytokines from SeNps-treated cells. Moreover, RAW246.7 macrophages exhibited a higher rate of phagocytosis against treated CT26 when compared to control cells. Taken together, our findings indicate that treatment with SeNps might be an efficient strategy to destroy tumour cells by inducing apoptotic cell death and triggering immune responses.
Γλώσσα:	Αγγλικά
Σελίδες:	22
DOI:	10.3390/cancers13215335
EISSN:	2072-6694
Θεματική κατηγορία:	[EL] Ιατρική Βιοτεχνολογία[EN] Medical Biotechnology [EL] Βιοχημεία και Μοριακή βιολογία[EN] Biochemistry and Molecular Biology [EL] Νανοεπιστήμη και Νανοτεχνολογία[EN] Nanoscience and Nanotechnology
Λέξεις-κλειδιά:	biogenic nanoparticles; selenium nanoparticles; SeNps; Lactobacillus casei; colon cancer; apoptosis; immunogenic cell death
Κάτοχος πνευματικών δικαιωμάτων:	© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Όροι και προϋποθέσεις δικαιωμάτων:	This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
Ηλεκτρονική διεύθυνση του τεκμηρίου στον εκδότη:	https://www.mdpi.com/2072-6694/13/21/5335
Ηλεκτρονική διεύθυνση περιοδικού:	https://www.mdpi.com/journal/cancers
Τίτλος πηγής δημοσίευσης:	Cancers
Τεύχος:	21
Τόμος:	13
Σελίδες τεκμηρίου (στην πηγή):	Article no 5335
Σημειώσεις:	This research is co-financed by Greece and the European Union (European Social FundESF) through the Operational Programme “Human Resources Development, Education and Lifelong Learning 2014–2020” in the context of the project ‘Biosynthesis of SeNps in probiotic lactobacilli and investigation of their anticancer and immunostimulatory activity’ (MIS 5050556).
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