Παρακαλώ χρησιμοποιήστε αυτό το αναγνωριστικό για να παραπέμψετε ή να δημιουργήσετε σύνδεσμο προς αυτό το τεκμήριο:
https://hdl.handle.net/123456789/1319| Τύπος: | Ανακοίνωση σε συνέδριο |
| Τίτλος: | Exosomes enter glial cells through an actin-network endocytic pathway mediatinge α-syn transmission |
| Συγγραφέας: | [EL] Πανταζοπούλου, Μαρίνα[EN] Pantazopoulou, Marina [EL] Λαμπροκωστοπούλου, Αγαρίστη[EN] Lamprokostopoulou, Agaristi [EL] Αλεξάκη, Αναστασία[EN] Alexaki, Anastasia [EL] Δελής, Αναστάσιος[EN] Delis, Anastasios [EL] Παγκάκης, Σταμάτης[EN] Pagakis, Stamatis [EL] Στεφανής, Λεωνίδας[EN] Stefanis, Leonidas [EL] Βεκρέλλης, Κώστας[EN] Vekrellis, Kostas |
| Ημερομηνία: | 15/03/2023 |
| Περίληψη: | Objective: Exosomes have recently emerged as key players in cellular communication in both physiological and pathological processes in the brain, particularly in synucleinopathies. However, the interplay between exosomes and the different cell types of the brain (neurons, astrocytes, microglia) has yet to be elucidated. The current study aims to examine the intracellular trafficking pathway of exosomes in primary neuronal and glial cells linked with the exosome- associated α-syn transmission. Methods: Mouse cortical neurons, microglia and astrocytic primary cultures were incubated with DiI-stained mouse brain-derived exosomes, in the absence or presence of recombinant fibrillar human α-Syn. The internalization and trafficking pathways were analysed by immunofluorescence in cells treated with pharmacological reagents that block the major endocytic pathways. Results: Brain-derived exosomes were internalized by both microglia and astrocytes but less efficiently by cortical neurons. Colocalization of exosomes with early and late endocytic markers (Rab5, Lamp1) indicated that exosomes are sorted to endolysosomes. Treatment with Cytochalasin D, that blocks actin-dependent phagocytosis and macropinocytosis, inhibited exosome entry into glial cells. Dynasore, that inhibits dynamin-dependent endocytosis, did not appear to affect exosome uptake, however the sorting to late endosomes was delayed. Exosome-associated fibrillar α-Syn was efficiently uptaken and detected within glial cells. Conclusions: Exosomes enter glial cells through an actin network-dependent phagocytic pathway and are sorted to endolysosomes for degradation or recycled back to the plasma membrane. Further, brain-derived exosomes are capable of mediating cell-to-glia transmission of pathological α-Syn that is also likely targeted to the endocytic pathway for clearance. |
| Γλώσσα: | Αγγλικά |
| Τόπος δημοσίευσης: | Barcelona, Spain |
| Σελίδες: | 1 |
| Θεματική κατηγορία: | [EL] Νευροεπιστήμες[EN] Neurosciences |
| Κάτοχος πνευματικών δικαιωμάτων: | © The Author(s) 2022 |
| Διατίθεται ανοιχτά στην τοποθεσία: | https://cslide.ctimeetingtech.com/adpd22/attendee/eposter/poster/998?r=cp8%7E20 |
| Όνομα εκδήλωσης: | International Conference on Alzheimer's and Parkinson's Diseases and related neurological disorders, AD/PD, 2022 |
| Τοποθεσία εκδήλωσης: | Barcelona, Spain |
| Ημ/νία έναρξης εκδήλωσης: | 15/03/2022 |
| Ημ/νία λήξης εκδήλωσης: | 20/03/2022 |
| Εμφανίζεται στις συλλογές: | Ερευνητικές ομάδες |
Αρχεία σε αυτό το τεκμήριο:
| Αρχείο | Περιγραφή | Σελίδες | Μέγεθος | Μορφότυπος | Έκδοση | Άδεια | |
|---|---|---|---|---|---|---|---|
| adpd abstract.png | abstract | 207.12 kB | image/png | Δημοσιευμένη/του Εκδότη |  | Δείτε/ανοίξτε |