1. Αποθετήριο Παραδοτέων Υποτρόφων Δράσεων ΕΣΠΑ Τριτοβάθμιας Εκπαίδευσης 2017-2023
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Τύπος:	Αναρτημένη ανακοίνωση (poster)
Τίτλος:	Investigation of the role of tumor suppressor CYLD in the differentiation of breast epithelial cells into adipocytes
Συγγραφέας:	[EL] Χάντουε, Πωλ[EN] Hadweh, Paul [EL] Γωνίδας, Χρήστος[EN] Gonidas, Christos [EL] Μόσιαλος, Γεώργιος[EN] Mosialos, George
Ημερομηνία:	15/04/2021
Περίληψη:	Metastatic breast cancer cells have been shown to transdifferentiate into growth arrested adipocytes. This capacity correlates with the plasticity of cells which have undergone epithelial-mesenchymal transition (EMT) and opens up new possibilities for therapeutic intervention. Inactivation or downregulation of the tumor suppressor CYLD induces EMT in mammary epithelial cells. Additionally, targeted inactivation of CYLD leads to lipid accumulation in mouse tissues. Based on these findings , we sought to investigate whether CYLD plays a role in the differentiation of breast cancer cells into adipocytes. For this purpose, the CRISPR/Cas9 technology was employed. Utilizing appropriate sgRNA sequences, monoclonal cell lines expressing or lacking expression of CYLD were derived from the MCF7 and MDA-MB-231 breast cancer cell lines. These derivative clones, as well as CYLD expressing and CYLD-null breast cancer cells MCF10A and HCC1937 previously generated at our laboratory, were subjected to adipogenic differentiation. After seeding and overnight incubation at 37oC 5% CO2, the cells were treated with 200ng/ml BMP2 for 3 days, 200ng/ml BMP2 and 2μM rosiglitazone for 4 days and 2μM rosiglitazone for 3 days. The cells were then stained with Oil Red O. After bright-field images were captured by a light microscope, the dye was extracted in isopropanol and its absorbance was determined at 500nm. The differentiation efficiency was limited, and no statistically significant differences were observed in the adipogenic capacity of control and CYLD-deficient cells. Our findings indicate that inactivation of CYLD is not sufficient to enhance adipogenic differentiation of mammary epithelial cells under the conditions that were applied based on the literature. Future studies will investigate additional adipogenic conditions as well as gene expression differences between control and CYLD-deficient mammary epithelial cells in order to better understand he role of CYLD in the homeostasis and differentiation of mammary epithelial cells.
Γλώσσα:	Αγγλικά
Τόπος δημοσίευσης:	Θεσσαλονίκη, Ελλάδα
Σελίδες:	6
Θεματική κατηγορία:	[EL] Βιοχημεία και Μοριακή βιολογία[EN] Biochemistry and Molecular Biology
Κάτοχος πνευματικών δικαιωμάτων:	© 2021 Christos Gonidas, Paul Hadweh, Geroge Mosialos
Όροι και προϋποθέσεις δικαιωμάτων:	This work is licensed under a Creative Commons Attribution 4.0 International License.
Όνομα εκδήλωσης:	Διαδικτυακό Συνέδριο Ελληνικής Εταιρείας Βιοχημείας & Μοριακής Βιολογίας “Regulation of gene expression and disease mechanisms”
Τοποθεσία εκδήλωσης:	Thessaloniki, Greece
Ημ/νία έναρξης εκδήλωσης:	15/04/2021
Ημ/νία λήξης εκδήλωσης:	15/04/2021
Σημειώσεις:	Συνέδρια-Σεμινάρια Ελληνικής Εταιρείας Βιοχημείας & Μοριακής Βιολογίας - https://eebmb.gr/index.php/news/events This research is co-financed by Greece and the European Union (European Social Fund- ESF) through the Operational Programme «Human Resources Development, Education and Lifelong Learning 2014-2020» in the context of the project “The role of tumor suppressor protein CYLD in mammary epithelial cell differentiation to adipocytes.” (MIS 5047914).”
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