1. Αποθετήριο Παραδοτέων Υποτρόφων Δράσεων ΕΣΠΑ Τριτοβάθμιας Εκπαίδευσης 2017-2023
2. Όλο το Αποθετήριο
3. Ερευνητικές ομάδες

Τύπος:	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Fast and sensitive determination of the fungicide carbendazim in fruit juices with an immunosensor based on white light reflectance spectroscopy
Συγγραφέας:	[EL] Κουκουβίνος, Γεώργιος[EN] Koukouvinos, Georgios [EL] Καραχάλιου, Χρυσούλα-Ευαγγελία[EN] Karachaliou, Chrysoula-Evangelia [EL] Ράπτης, Ιωάννης[EN] Raptis, Ioannis [EL] Πέτρου, Παναγιώτα[EN] Petrou, Panagiota [EL] Λιβανίου, Ευαγγελία[EN] Livaniou, Evangelia [EL] Κακαμπάκος, Σωτήρης[EN] Kakabakos, Sotirios
Ημερομηνία:	13/05/2021
Περίληψη:	Abstract: Carbendazim is a systemic benzimidazole-type fungicide with broad-spectrum activity against fungi that undermine food products safety and quality. Despite its effectiveness, carbendazim constitutes a major environmental pollutant, being hazardous to both humans and animals. Therefore, fast and reliable determination of carbendazim levels in water, soil, and food samples is of high importance for both food industry and public health. Herein, an optical biosensor based on white light reflectance spectroscopy (WLRS) for fast and sensitive determination of carbendazim in fruit juices is presented. The transducer is a Si/SiO2 chip functionalized with a benzimidazole conjugate, and determination is based on a competitive immunoassay format. Thus, for the assay, a mixture of an in-house developed rabbit polyclonal anti-carbendazim antibody with the standards or samples is pumped over the chip, followed by biotinylated secondary antibody and streptavidin. The WLRS platform allows for real-time monitoring of biomolecular interactions carried out onto the Si/SiO2 chip by transforming the shift in the reflected interference spectrum caused by the immunoreaction to effective biomolecular adlayer thickness. The sensor is able to detect 20 ng/mL of carbendazim in fruit juices with high accuracy and precision (intra- and inter-assay CVs < 6.9% and < 9.4%, respectively) in less than 30 min, applying a simple sample treatment that alleviates any “matrix-effect” on the assay results and a 60 min preincubation step for improving assay sensitivity. Excellent analytical characteristics and short analysis time along with its small size render the proposed WLRS immunosensor ideal for future on-the-spot determination of carbendazim in food and environmental samples.
Γλώσσα:	Αγγλικά
Σελίδες:	16
DOI:	10.3390/bios11050153
EISSN:	2079-6374
Θεματική κατηγορία:	[EL] Άλλες φυσικές επιστήμες[EN] Other natural sciences
Λέξεις-κλειδιά:	white light reflectance spectroscopy; real-time immunosensor; ELISA; pesticides; carbendazim; fruit juices
Κάτοχος πνευματικών δικαιωμάτων:	© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Όροι και προϋποθέσεις δικαιωμάτων:	This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
Ηλεκτρονική διεύθυνση του τεκμηρίου στον εκδότη:	https://www.mdpi.com/2079-6374/11/5/153
Ηλεκτρονική διεύθυνση περιοδικού:	https://www.mdpi.com/journal/biosensors
Τίτλος πηγής δημοσίευσης:	Biosensors
Τεύχος:	5
Τόμος:	11
Σελίδες τεκμηρίου (στην πηγή):	Article no 153
Σημειώσεις:	This research is cofinanced by Greece and the European Union (European Social Fund— ESF) through the Operational Program “Human Resources Development, Education and Lifelong Learning 2014–2020” in the context of the project “Development of immunochemical analytical methodology, especially an ELISA and a biosensor assay, for determining benzimidazole-type pesticides” (MIS 5047811).
Εμφανίζεται στις συλλογές:	Ερευνητικές ομάδες