1. Αποθετήριο Παραδοτέων Υποτρόφων Δράσεων ΕΣΠΑ Τριτοβάθμιας Εκπαίδευσης 2017-2023
2. Όλο το Αποθετήριο
3. Μεταδιδακτορικοί ερευνητές

Τύπος:	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Paper-based microfluidic device with integrated sputtered electrodes for stripping voltammetric determination of DNA via Quantum Dot labeling
Συγγραφέας:	[EL] Κόκκινος, Χρήστος[EN] Kokkinos, Christos [EL] Γκιώκας, Δημοσθένης[EN] Giokas, Dimosthenis [EL] Οικονόμου, Αναστάσιος[EN] Economou, Anastasios [EL] Πέτρου, Παναγιώτα[EN] Petrou, Panagiota [EL] Κακαμπάκος, Σωτήριος[EN] Kakabakos, Sotirios
Ημερομηνία:	18/12/2017
Περίληψη:	This work reports a microfabricated electrochemical paper-based analytical device (ePAD) for the voltammetric determination of DNA. The device is patterned by wax-printing on paper and features a circular assay zone connected to an inlet zone and a sink via grooved microfluidic channels for accelerated flow rate. An electrochemical cell with integrated electrodes is formed on the reverse side of the paper by sputtering of thin metal films (Sn, Pt and Ag as the working, counter and reference electrode, respectively). Proof-of-principle of the ePAD for biosensing is demonstrated for a DNA assay involving attachment of capture DNA, hybridization with biotinylated target oligonucleotide and labeling with streptavidin-conjugated CdSe/ZnS quantum dots (QDs). After the acidic dissolution of the QDs, the released Cd(II) is quantified by anodic stripping voltammetry (ASV) at the Sn-film working electrode. Thanks to the synergistic effects of QDs amplification, the inherent sensitivity of ASV and the excellent detection capabilities of the Sn-film working electrode for Cd(II), the target DNA can be detected at levels as low as 0.11 pmol L−1 using sample volumes as low as 1 μL. The developed microfluidic ePAD costs only 0.11$ and presents favorable fabrication and operational features that make it an excellent candidate biosensor for simple and ultrasensitive point-of-need testing.
Γλώσσα:	Αγγλικά
Σελίδες:	6
DOI:	10.1021/acs.analchem.7b04274
ISSN:	0003-2700
Θεματική κατηγορία:	[EL] Αναλυτική χημεία[EN] Analytical Chemistry
Λέξεις-κλειδιά:	sensors
Κάτοχος πνευματικών δικαιωμάτων:	© 2017 American Chemical Society
Όροι και προϋποθέσεις δικαιωμάτων:	καμία σημείωση
Ηλεκτρονική διεύθυνση του τεκμηρίου στον εκδότη:	https://pubs.acs.org/doi/10.1021/acs.analchem.7b04274#
Ηλεκτρονική διεύθυνση περιοδικού:	https://pubs.acs.org/journal/ancham
Τίτλος πηγής δημοσίευσης:	Analytical Chemistry
Τεύχος:	2
Τόμος:	90
Σελίδες τεκμηρίου (στην πηγή):	1092–1097
Σημειώσεις:	The research project was implemented under the IKY Scholarship which was funded by the Action “Strengthening Postdoctoral Researchers” act from the resources of EP “Human Resources Development Program, Education and Lifelong Learning” with priority axes 6,8,9 and is cofinanced by the European Social Fund (ESF) and the Greek Government.
Εμφανίζεται στις συλλογές:	Μεταδιδακτορικοί ερευνητές