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https://hdl.handle.net/123456789/365| Τύπος: | Άρθρο σε επιστημονικό περιοδικό |
| Τίτλος: | Multidrug resistance of Penicillium expansum to fungicides: whole transcriptome analysis of MDR strains reveals overexpression of efflux transporter genes |
| Εναλλακτικός τίτλος: | Ανθεκτικότητα πληθυσμών του φυτοπαθογόνου μύκητα Penicillium expansum σε μυκητοκτόνα και τρανσκριπτομική διερεύνηση του μηχανισμού πολλαπλής ανθεκτικότητας (MDR) |
| Συγγραφέας: | [EL] Καραογλανίδης, Γεώργιος[EN] Karaoglanidis, Georgios |
| Συντελεστής: | [EL] Σαμαράς, Αναστάσιος[EN] Samaras, Αnastasios [EL] Ντάσιου, Παναγιώτα[EN] Ntasiou, Panagiota [EL] Μυρεσιώτης, Χαράλαμπος[EN] Myresiotis, Charalampos |
| Ημερομηνία: | 29/09/2020 |
| Περίληψη: | Penicillium expansum is the most common apple fruit postharvest spoilage agent that causes a disease known as Blue Mold. Disease control is based on fungicide use. However, development of resistance to fungicides hampers the success of this control method. Fungicide sensitivity monitoring studies in Greece revealed the presence of pathogen strains exhibiting simultaneous resistance to different chemically unrelated compounds (multidrug resistance, MDR). This study was initiated aiming primarily to test the hypothesis that the MDR phenotype is associated with overexpression of efflux transporter genes and to determine the fitness of the MDR isolates. The monitoring study (n = 264) and the measurements of sensitivity in terms of EC50 values to 9 different compounds revealed that almost 5% of the population was of the MDR type. In the selected MDR isolates, the highest resistant factors were calculated for fludioxonil and pyraclostrobin, while the same isolates were moderately resistant to cyprodinil, thiophanate methyl and fluxapyroxad. In the resistant strains no target site mutations were detected in the target genes of each fungicide class, while in addition, a synergistic activity was observed between fungicides and the drug transporter modulator verapamil in some isolates. To obtain a direct insight on the resistance mechanism, the transcriptome of 2 MDR and 1 sensitive isolates was sequenced using Illumina HiSeq 2500 and differences in efflux transporter gene expression profile were figured out. Gene expression profiling analysis was performed before and after the exposure of fungal mycelia to fludioxonil. This analysis revealed the up-regulation of several MFS transporter genes and a limited number of ABC transporter genes either before or after the exposure to fludioxonil in the MDR isolates. Expression results for genes with the highest expression levels were verified by qRT-PCR assays. Fitness components measurements revealed that MDR isolates were of lower mycelial growth and pathogenicity compared to sensitive strains but they were producing higher number of conidia. The above mentioned data represent the first report of MDR in P. expansum associated with overexpression of drug efflux transporters and contribute to our knowledge in the mechanisms associated with fungicide resistance development in this fungal species. |
| Γλώσσα: | Αγγλικά |
| Σελίδες: | 15 |
| DOI: | 10.1016/j.ijfoodmicro.2020.108896 |
| ISSN: | 0168-1605 |
| Θεματική κατηγορία: | [EL] Άλλες Γεωπονικές Επιστήμες[EN] Other Agricultural Sciences |
| Λέξεις-κλειδιά: | Penicillium expansum; Multidrug resistance; Fungicide resistance; RNA-seq; MFS-transporters; ABC-transporters; Apple fruit |
| Κάτοχος πνευματικών δικαιωμάτων: | © 2020 Elsevier B.V. All rights reserved. |
| Ηλεκτρονική διεύθυνση του τεκμηρίου στον εκδότη: | https://www.sciencedirect.com/science/article/abs/pii/S0168160520303901?via%3Dihub |
| Ηλεκτρονική διεύθυνση περιοδικού: | https://www.sciencedirect.com/journal/international-journal-of-food-microbiology |
| Τίτλος πηγής δημοσίευσης: | International Journal of Food Microbiology |
| Τεύχος: | 16 December 2020 |
| Τόμος: | 335 |
| Σελίδες τεκμηρίου (στην πηγή): | Article no 108896 |
| Σημειώσεις: | This research is co-financed by Greece and the European Union (European Social Fund-ESF) through the Operational Programme “Human Resources Development, Education and Lifelong Learning 2014–2020” in the context of the project “Resistance of Penicillium expansum populations to fungicides and transcriptomic investigation of the multidrug resistance mechanism (MDR)” (MIS 5004852). |
| Εμφανίζεται στις συλλογές: | Ερευνητικές ομάδες |
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