1. Αποθετήριο Παραδοτέων Υποτρόφων Δράσεων ΕΣΠΑ Τριτοβάθμιας Εκπαίδευσης 2017-2023
2. Όλο το Αποθετήριο
3. Ερευνητικές ομάδες

Τύπος:	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Biocatalyst potential of cellulose-degrading microorganisms isolated from orange juice processing waste
Συγγραφέας:	[EL] Ζέρβα, Ιωάννα[EN] Zerva, Ioanna [EL] Ρέμμας, Νικόλαος[EN] Remmas, Nikolaos [EL] Ντούγιας, Σπυρίδων[EN] Ntougias, Spyridon
Ημερομηνία:	02/03/2019
Περίληψη:	Cellulases can be applied as macerating and peeling enzymes in the orange juice processing industry. In this work, indigenous cellulose-degrading microorganisms were isolated from orange juice processing waste through successive enrichment procedures using carboxymethyl cellulose (CMC) as the sole carbon source. A total of 24 microbial isolates were screened for their ability to grow in CMC liquid medium, resulting in the selection of seven isolates. The latter were further assessed by determining their endo-1,4-β-d-glucanase, exo-1,4-β-d-glucanase, and β-1,4-d-glucosidase activities, of which their respective activities were as high as 3.89, 10.67, and 10.69 U/mg protein. All cellulose-degraders selected belonged to the genus Paenibacillus, although to distinct operational taxonomic units related to P. xylanexedens, P. tundrae, and P. pabuli (operational taxonomic unit—OTU#1) and to P. wynnii, P. odorifer, and P. donghaensis (OTU#2) spectrum. Regarding the cellulase activities of the orange juice processing waste, endo-1,4-β-d-glucanase activity (4.00 ± 0.11 U/g) was exerted only extracellularly, whereas exo-1,4-β-d-glucanase (2.60 ± 0.19 U/g) and β-1,4-d-glucosidase (5.69 ± 0.23 U/g) activities were exhibited both extracellularly and intracellularly. In conclusion, orange juice processing waste can be considered as a valuable source for the isolation of cellulose-degrading microbiota with potential uses in beverage industry, solid state fermentation and energy production.
Γλώσσα:	Αγγλικά
Σελίδες:	11
DOI:	10.3390/beverages5010021
ISSN:	2306-5710
Θεματική κατηγορία:	[EL] Επιστήμες Μηχανικού και Τεχνολογία[EN] Engineering and Technology
Λέξεις-κλειδιά:	cellulolytic bacteria; Cellulase; glucanase; glucosidase; beverage industry; solid state fermentation; Paenibacillus
Κάτοχος πνευματικών δικαιωμάτων:	© 2019 by the authors. Licensee MDPI, Basel, Switzerland
Όροι και προϋποθέσεις δικαιωμάτων:	This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Διατίθεται ανοιχτά στην τοποθεσία:	https://www.mdpi.com/2306-5710/5/1/21/htm
Ηλεκτρονική διεύθυνση του τεκμηρίου στον εκδότη:	https://www.mdpi.com/2306-5710/5/1/21
Ηλεκτρονική διεύθυνση περιοδικού:	https://www.mdpi.com/journal/beverages
Τίτλος πηγής δημοσίευσης:	Beverages
Τεύχος:	1
Τόμος:	5
Σελίδες τεκμηρίου (στην πηγή):	Article no 21
Σημειώσεις:	(This article belongs to the Special Issue Novel Beverages and Novel Technologies for Their Production) This research was implemented through the Operational Program “Human Resources Development, Education and Lifelong Learning”, code MIS (OPS) 5006203, and was co-financed by the European Union (European Social Fund) and Greek national funds
Εμφανίζεται στις συλλογές:	Ερευνητικές ομάδες