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https://hdl.handle.net/123456789/406| Τύπος: | Άρθρο σε επιστημονικό περιοδικό |
| Τίτλος: | Study of fecal and urinary metabolite perturbations induced by chronic ethanol treatment in mice by UHPLC-MS/MS targeted profiling |
| Συγγραφέας: | [EL] Δέδα, Όλγα[EN] Deda, Olga [EL] Βιργιλίου, Χριστίνα[EN] Virgiliou, Christina [EL] Ορφανίδης, Αμβρόσιος[EN] Orfanidis, Amvrosios [EL] Γκίκα, Ελένη[EN] Gika, Helen |
| Ημερομηνία: | 16/10/2019 |
| Περίληψη: | Alcoholic liver disease (ALD) as a consequence of ethanol chronic consumption could lead to hepatic cirrhosis that is linked to high morbidity and mortality. Disease diagnosis is still very challenging and usually clear findings are obtained in the later stage of ALD. The profound effect of ethanol on metabolism can be depicted using metabolomics; thus, the discovery of novel biomarkers could shed light on the initiation and the progression of the ALD, serving diagnostic purposes. In the present study, Hydrophilic Interaction Liquid Chromatography tandem Mass Spectrometry HILIC-MS/MS based metabolomics analyisis of urine and fecal samples of C57BL/6 mice of both sexes at two sampling time points was performed, monitoring the effect of eight-week ethanol consumption. The altered hepatic metabolism caused by ethanol consumption induces extensive biochemical perturbations and changes in gut microbiota population on a great scale. Fecal samples were proven to be a suitable specimen for studying ALD since it was more vulnerable to the metabolic changes in comparison to urine samples. The metabolome of male mice was affected on a greater scale than the female metabolome due to ethanol exposure. Precursor small molecules of essential pathways of energy production responded to ethanol exposure. A meaningful correlation between the two studied specimens demonstrated the impact of ethanol in endogenous and symbiome metabolism |
| Γλώσσα: | Αγγλικά |
| Σελίδες: | 16 |
| DOI: | 10.3390/metabo9100232 |
| ISSN: | 2218-1989 |
| Θεματική κατηγορία: | [EL] Ιατρική και Επιστήμες Υγείας[EN] Medical and Health Sciences [EL] Αναλυτική χημεία[EN] Analytical Chemistry |
| Λέξεις-κλειδιά: | ethanol; alcohol; toxicity; metabolomics; feces; urine; ALD; mice |
| Κάτοχος πνευματικών δικαιωμάτων: | © 2019 by the authors. Licensee MDPI |
| Όροι και προϋποθέσεις δικαιωμάτων: | This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
| Διατίθεται ανοιχτά στην τοποθεσία: | https://www.mdpi.com/2218-1989/9/10/232 |
| Ηλεκτρονική διεύθυνση του τεκμηρίου στον εκδότη: | https://www.mdpi.com/2218-1989/9/10/232 |
| Ηλεκτρονική διεύθυνση περιοδικού: | https://www.mdpi.com/journal/metabolites |
| Τίτλος πηγής δημοσίευσης: | Metabolites |
| Τεύχος: | 10 |
| Τόμος: | 9 |
| Σελίδες τεκμηρίου (στην πηγή): | Article no 232 |
| Σημειώσεις: | This research is carried out/funded in the context of the project “Study of alcohol toxicity at chronic and acute alcohol consumption, discovery and evaluation of biomarkers by applying metabolomics based methods” (5005029) under the call for proposals “Supporting researchers with emphasis on new researchers” (EDULLL 34). The project is co-financed by Greece and the European Union (European Social Fund (ESF)) by the Operational Programme Human Resources Development, Education and Lifelong Learning 2014–2020. |
| Εμφανίζεται στις συλλογές: | Ερευνητικές ομάδες |
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