1. Αποθετήριο Παραδοτέων Υποτρόφων Δράσεων ΕΣΠΑ Τριτοβάθμιας Εκπαίδευσης 2017-2023
2. Όλο το Αποθετήριο
3. Ερευνητικές ομάδες

Τύπος:	Άρθρο σε περιοδικό
Τίτλος:	Lack of macroscopically evident cardiac regeneration or spontaneous functional recovery in infarcted neonatal pigs
Εναλλακτικός τίτλος:	Μελέτη της ικανότητας της νεογνικής χοίρειας καρδιάς για ενδογενή μυοκαρδιακή αναγέννηση
Συγγραφέας:	[EL] Μαλλιάρας, Κωνσταντίνος[EN] Malliaras, Konstantinos [EL] Πολυχρονοπούλου, Εριέττα[EN] Polychronopoulou, Erietta [EL] Πουλακίδα, Ειρήνη[EN] Poulakida, Eirini [EL] Σαγρής, Δημήτριος[EN] Sagris, Dimitrios [EL] Μακαρίτσης, Κωνσταντίνος[EN] Makaritsis, Konstantinos
Ημερομηνία:	15/11/2019
Περίληψη:	The adult mammalian heart harbors a traceable -albeit limited capacity for endogenous cardiomyocyte replenishment throughout life. Given that heart disease comprises a major cause of mortality, the elucidation of the innate cardiac regenerative mechanisms is of paramount therapeutic importance; exogenous stimulation of said mechanisms would potentially enable iatrogenic regeneration in diseased hearts. In contrast to the adult mammalian heart, the neonatal murine heart exhibits a remarkable capacity for spontaneous regeneration following cardiac injury. Whether such robust cardiac regenerative potential is also present in neonatal large mammals has been under-investigated. Recently, two studies reported that 1- and 2-day-old neonatal pigs exhibit a robust cardiac regenerative response post-myocardial infarction (MI), characterized by minimal scarring and spontaneous, complete recovery of left ventricular (LV) function. This regenerative capacity is purportedly lost after the first 2 days of life; neonatal pigs infarcted on postnatal day 3 (P3) or later developed mature scars and exhibited persistent systolic dysfunction. Here, we also investigated the regenerative potential of neonatal porcine hearts post-MI. Twelve farm piglets were randomized to undergo MI by permanent ligation of the left anterior descending artery on P1 or P3. Piglets were euthanized at 7weeks post-MI. Transthoracic echocardiography was performed just prior to euthanasia. Explanted hearts underwent staining with triphenyl-tetrazolium chloride and Masson's Trichrome for scar assessment. contrary to recently published reports, we did not observe a robust cardiac regenerative response in neonatal P1 porcine hearts post-MI. Specifically, while previous studies reported minimal scarring and complete recovery of LV function in infarcted P1 and P2 (but not P3) piglets, all infarcted hearts of P1 piglets in our study exhibited substantial scarring and persistent hypokinesia of the infarcted myocardium. Scars were similar morphometrically (in terms of infarct size, circumference, and transmurality) and qualitatively (consisting of dense collagen fibers) in P1 and P3 piglets. Furthermore, the degree of systolic dysfunction was comparable in P1 and P3 piglets. While we cannot exclude that increased neomyogenesis may occur in infarcted P1 piglets at a microscopic level, a robust, clinically relevant regenerative response would result in decreased infarct size and improved cardiac regional systolic function in P1 piglets compared to P3 piglets. Because none of these indirect indices of robust neomyogenesis were observed, we conclude that macroscopically evident regeneration does not occur in infarcted P1 porcine hearts.
Γλώσσα:	Αγγλικά
Σελίδες:	3
DOI:	10.1016/j.hjc.2019.10.012
ISSN:	1109-9666
Θεματική κατηγορία:	[EL] Καρδιολογία και Καρδιοαγγειακά νοσήματα[EN] Cardiology and Cardiovascular Medicine
Λέξεις-κλειδιά:	μυοκαρδιακή αναγέννηση
Κάτοχος πνευματικών δικαιωμάτων:	© 2019 Hellenic Society of Cardiology. Publishing services by Elsevier B.V.
Όροι και προϋποθέσεις δικαιωμάτων:	This is an open access article under the CC BY-NC-ND license (http://creativecommons. org/licenses/by-nc-nd/4.0/).
Διατίθεται ανοιχτά στην τοποθεσία:	https://www.sciencedirect.com/science/article/pii/S1109966619302891
Ηλεκτρονική διεύθυνση του τεκμηρίου στον εκδότη:	https://www.sciencedirect.com/science/article/pii/S1109966619302891
Ηλεκτρονική διεύθυνση περιοδικού:	https://www.sciencedirect.com/journal/hellenic-journal-of-cardiology
Τίτλος πηγής δημοσίευσης:	Hellenic Journal of Cardiology
Τεύχος:	3
Τόμος:	61
Σελίδες τεκμηρίου (στην πηγή):	219-221
Σημειώσεις:	This research was co-financed by Greece and the European Union (European Social Fund- ESF) through the Operational Program «Human Resources Development, Education and Lifelong Learning 2014-2020» in the context of the project “Study of the capacity of the neonatal porcine heart for endogenous myocardial regeneration” (MIS 5005736).
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