1. Αποθετήριο Παραδοτέων Υποτρόφων Δράσεων ΕΣΠΑ Τριτοβάθμιας Εκπαίδευσης 2017-2023
2. Όλο το Αποθετήριο
3. Ερευνητικές ομάδες

Τύπος:	Αναρτημένη ανακοίνωση (poster)
Τίτλος:	In vitro comparison of the effects of “reduced risk” nicotine products and of cigarette smoke on adipocyte survival and differentiation
Εναλλακτικός τίτλος:	In vitro συγκριτική μελέτη της επίδρασης των προϊόντων "μειωμένου κινδύνου" για τη νικοτίνη και του τσιγάρου στη βιωσιμότητα και διαφοροποίηση των λιποκυττάρων
Συγγραφέας:	[EN] El Mubarak, Mohamed - Ahmed [EL] Ζαγορίτη, Ζωή[EN] Zagoriti, Zoi [EL] Φαρσαλινός, Κωνσταντίνος[EN] Farsalinos, Konstantinos [EL] Τοπούζης, Σταύρος[EN] Topouzis, Stavros
Ημερομηνία:	29/05/2019
Περίληψη:	Background: Cigarette smoking (CS) has been implicated in cardiovascular, metabolic and respiratory diseases and it constitutes a major cause of lung and other cancers in humans. In the adipose tissue, nicotine has been reported to induce lipolysis, leading to body weight loss1 , while CS has been associated with insulin resistance and hyperinsulinemia2,3. Electronic cigarettes (e-cig) and heated tobacco products have been designed to deliver nicotine in a vaping solution or aerosol, without tobacco combustion. Thus, they are assumed to be safer alternatives to conventional cigarettes. In the present study, we evaluate the cytotoxic effects of CS, e-cig vapor and heated tobacco aerosol, as well as the impact of the contained nicotine on the differentiation of 3T3-L1 pre-adipocytes to “beige” adipocytes. Methods: By using a commercially available e-cig device and a set of impingers, 327 mg of e-liquid containing 1.2% w/w nicotine and no flavor were evaporated and extracted in 40 mL of culture medium DMEM. Similarly, extracts of three cigarettes and four heated tobacco sticks in DMEM were also produced. LC/MS-MS was applied to determine the levels of nicotine in each extract. Dilutions of these extracts were administered to 3T3-L1 pre-adipocyte cultures and cytotoxicity was measured by the MTT assay. The differentiation of 3T3-L1 pre-adipocytes to beige adipocytes was obtained in the presence of differentiation inducers, as described previously4 . The cells were exposed to either nicotine (1 or 3 ug/mL) or CS extract dilutions (1% and 10%), throughout the differentiation process. The differentiation efficiency and adipocytic phenotype were assessed by Oil Red O staining and by analyzing the expression of marker genes characteristic of brown adipose tissue by RT-PCR. Results: The quantification of nicotine in the media extracts showed that CS and heated tobacco extracts contained 110 ug/mL each, while the e-cig extract contained 60 ug/mL of nicotine. No cytotoxic effects of CS extract dilutions below 10% were observed after 24h and 48h of exposure of the 3T3-L1 cells. The effect of the extracts and of nicotine on 3T3-L1 preadipocyte differentiation to beige adipocytes is currently being evaluated.
Γλώσσα:	Αγγλικά
Τόπος δημοσίευσης:	Αθήνα, Ελλάδα
Σελίδες:	1
Θεματική κατηγορία:	[EL] Βιολογία[EN] Biological sciences
Λέξεις-κλειδιά:	cigarette; electronic cigarette; heated tobacco product; beige adipocytes
Κάτοχος πνευματικών δικαιωμάτων:	© by the authors
Διατίθεται ανοιχτά στην τοποθεσία:	http://www.nosmokesummit.org/wp-content/uploads/2019/06/2ndSummit_Abstract_Book.pdf
Ηλεκτρονική διεύθυνση του τεκμηρίου στον εκδότη:	http://www.nosmokesummit.org/wp-content/uploads/2019/06/2ndSummit_Abstract_Book.pdf
Τίτλος πηγής δημοσίευσης:	2nd Scientific Summit Tobacco Harm Reduction Abstract Book
Σελίδες τεκμηρίου (στην πηγή):	34-35
Όνομα εκδήλωσης:	2nd Scientific Summit Tobacco Harm Reduction: Novel Products, Research and Policy
Τοποθεσία εκδήλωσης:	Κέντρο Πολιτισμού Ίδρυμα Σταύρος Νιάρχος, Αθήνα, Ελλάδα
Ημ/νία έναρξης εκδήλωσης:	29/05/2019
Ημ/νία λήξης εκδήλωσης:	30/05/2019
Σημειώσεις:	No 13 in Abstract Book of 2nd Scientific Summit Tobacco Harm Reduction: Novel products, Research and Policy - http://2019.nosmokesummit.org/ The research/project “Reduced risk nicotine products: Comparative studies of activity in respiratory and adipose tissues” 80534 is implemented through/has been co-financed by the Operational Program “Human Resources Development, Education and Lifelong Learning” and is co-financed by the European Union (European Social Fund) and Greek national funds.
Εμφανίζεται στις συλλογές:	Ερευνητικές ομάδες