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https://hdl.handle.net/123456789/659| Τύπος: | Άρθρο σε επιστημονικό περιοδικό |
| Τίτλος: | Com1 as a promising protein for the differential diagnosis of the two forms of Q fever |
| Συγγραφέας: | [EL] Βρανάκης, Ιωσήφ[EN] Vranakis, Iosif [EL] Μαθιουδάκη, Ειρήνη[EN] Mathioudaki, Eirini [EL] Κοκκίνη, Σοφία[EN] Kokkini, Sofia [EL] Ψαρουλάκη, Άννα[EN] Psaroulaki, Anna |
| Ημερομηνία: | 18/11/2019 |
| Περίληψη: | : Coxiella burnetii is the causative agent of acute and chronic Q fever in humans. Although the isolates studied so far showed a difference in virulence potential between those causing the two forms of the disease, implying a difference in their proteomic profile, the methods used so far to diagnose the two forms of the disease do not provide sufficient discriminatory capability, and human infections may be often misdiagnosed. The aim of the current study was to identify the outer membrane Com1 (CBU_1910) as a candidate protein for serodiagnostics of Q fever. The protein was cloned, expressed, purified, and used as an antigen in ELISA. The protein was then used for the screening of sera from patients suffering from chronic Q fever endocarditis, patients whose samples were negative for phase I immunoglobulin G (IgG), patients for whom at least one sample was positive for phase I IgG, and patients suffering from any kind of rheumatoid disease. Blood donors were used as the control group. Following statistical analysis, 92.4% (122/132) of the samples tested agreed with the negative clinical diagnosis, and 72.2% (26/36) agreed with the positive clinical diagnosis. Moreover, a significant correlation to the presence of the disease (p = 0.00) was calculated. The results support the idea that a Com1 antigen-based serodiagnostic test may be useful for differential diagnosis of chronic Q fever. Further studies are required to compare more immunogenic proteins of the bacterium against samples originating from patients suffering from different forms of the disease. |
| Γλώσσα: | Αγγλικά |
| Σελίδες: | 12 |
| DOI: | 10.3390/pathogens8040242 |
| EISSN: | 2076-0817 |
| Θεματική κατηγορία: | [EL] Άλλες Ιατρικές Επιστήμες[EN] Other Medical Sciences |
| Λέξεις-κλειδιά: | Com1; Coxiella burnetii; antigenic; Q fever; ELISA |
| Κάτοχος πνευματικών δικαιωμάτων: | © 2019 by the authors. Licensee MDPI |
| Όροι και προϋποθέσεις δικαιωμάτων: | This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
| Διατίθεται ανοιχτά στην τοποθεσία: | https://www.mdpi.com/2076-0817/8/4/242 |
| Ηλεκτρονική διεύθυνση του τεκμηρίου στον εκδότη: | https://www.mdpi.com/2076-0817/8/4/242 |
| Ηλεκτρονική διεύθυνση περιοδικού: | https://www.mdpi.com/journal/pathogens |
| Τίτλος πηγής δημοσίευσης: | Pathogens |
| Τεύχος: | 4 |
| Τόμος: | 8 |
| Σελίδες τεκμηρίου (στην πηγή): | Article no 242 |
| Σημειώσεις: | The project “Protein testing for the differentiation of C. burnetii in livestock and human animals” of the Action “Supporting researchers with emphasis on young researchers” is implemented through the Operational Program “Human Resources Development, Education, and Lifelong Learning” and is co-financed by the European Union (European Social Fund) and Greek national funds. |
| Εμφανίζεται στις συλλογές: | Ερευνητικές ομάδες |
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