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https://hdl.handle.net/123456789/662| Τύπος: | Αναρτημένη ανακοίνωση (poster) |
| Τίτλος: | Patien-specific dosimetry for pediatric patients undergoing interventional procedures |
| Συγγραφέας: | [EL] Σπυράτου, Ελλάς[EN] Spyratou, Ellas [EL] Σούλη, Μαρία[EN] Souli, Maria [EL] Σεϊμένης, Ιωάννης[EN] Seimenis, Ioannis |
| Ημερομηνία: | 17/10/2019 |
| Περίληψη: | Objectives: Atomic Force Microscopy (AFM) is a scanning probe microscope which enables directly the non-invasive imaging of biological structures at a resolution of nanometer scale without requiring special sample treatments. Thus, it is ideal for the visualizing of DNA molecules. The size and distribution analysis of DNA breaks induced by ionizing radiation is of great importance and up-to-date mostly used techniques are based on gel electrophoresis or elution of DNA. However, these techniques are limited by their inability to resolve individual DNA fragments produced when more than one Double Strand Breaks (DSB) are induced along individual DNA molecules. Experiments have shown that the distribution profiles of DNA fragments differ significantly from random DSB model predictions used for the interpretation of the results. In this study, AFM was used to visualize directly the DNA fragmentation and quantify DNA breaks by using an in house algorithm. Methods: Plasmid DNA in solution with radical oxygen species scavenger is chosen as a simple model system of a cell. DNA damage was induced by pencil proton beam irradiation at different doses of 0-10 Gy in the position of the plateau of spread out Bragg Peak of a proton treatment plan (<200 MeV). Plasmid DNA samples diluted in MgCl2 were deposited on mica surface for AFM analysis. The length of each DNA molecule was measured by applying an in house algorithm implemented in Matlab. Results: The topological conformation of supercoiled, relaxed and linear DNA molecules was captured by using AFM. The images of irradiated DNA molecules were evaluated to derive fragment length distributions and to determine the average number of DSBs as a function of dose. The dose-effect curve reveals a non-linear relationship. Conclusions: AFM was employed successfully for direct observation of individual DNA molecules. It was experimentally determined that the DNA fragmentation is dosed dependent. |
| Γλώσσα: | Αγγλικά |
| Τόπος δημοσίευσης: | Ηράκλειο Κρήτης |
| Σελίδες: | 10 |
| Θεματική κατηγορία: | [EL] Βασική Ιατρική[EN] Basic Medicine [EL] Εφαρμοσμένη φυσική[EN] Applied Physics |
| Λέξεις-κλειδιά: | Atomic Force Microscopy; DNA damage; dosimetry |
| Κάτοχος πνευματικών δικαιωμάτων: | © by the author(s) |
| Όνομα εκδήλωσης: | XVII Balcan Congress of Radiology |
| Τοποθεσία εκδήλωσης: | Heraklion, Greece |
| Ημ/νία έναρξης εκδήλωσης: | 17/10/2019 |
| Ημ/νία λήξης εκδήλωσης: | 19/10/2019 |
| Σημειώσεις: | Congress site :http://www.bcr2019.gr/ This research is co-financed by Greece and the European Union (European Social Fund-ESF) through the Operational Programme “Human Resources Development, Education and Lifelong Learning 2014-2020” under the call for proposals “Supporting researchers with emphasis on new researchers” (EDULLL 34), in the context of the project “Patient-specific dosimetry for paediatric patients undergoing interventional cardiology procedures” (82089-MIS 5006356). |
| Εμφανίζεται στις συλλογές: | Ερευνητικές ομάδες |
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