Παρακαλώ χρησιμοποιήστε αυτό το αναγνωριστικό για να παραπέμψετε ή να δημιουργήσετε σύνδεσμο προς αυτό το τεκμήριο:
https://hdl.handle.net/123456789/812| Τύπος: | Άρθρο σε επιστημονικό περιοδικό |
| Τίτλος: | Targeted somatic mutagenesis through CRISPR/Cas9 ribonucleoprotein complexes in the olive fruit fly, Bactrocera oleae |
| Συγγραφέας: | [EN] Meccariello, Angela [EL] Τσουμάνη, Κωνσταντίνα[EN] Tsoumani, Konstantina [EN] Gravina, Andrea [EN] Primo, Pasquale [EN] Buonanno, Martina [EL] Ματθιόπουλος, Κώστας[EN] Mathiopoulos, Kostas [EN] Saccone, Giuseppe |
| Ημερομηνία: | 25/02/2020 |
| Περίληψη: | The olive fruit fly, Bactrocera oleae (Diptera: Tephritidae), is the most destructive insect pest of olive cultivation, causing significant economic and production losses. Here, we present the establishment of the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 methodology for gene disruption in this species. We performed targeted mutagenesis of the autosomal gene white (Bo‐we), by injecting into early embryos in vitro preassembled and solubilized Cas9 ribonucleoprotein complexes loaded with two gene‐specific single‐guide RNAs. Gene disruption of Bo‐we led to somatic mosaicism of the adult eye color. Large eye patches or even an entire eye lost the iridescent reddish color, indicating the successful biallelic mutagenesis in somatic cells. Cas9 induced either indels in each of the two simultaneously targeted Bo‐we sites or a large deletion of the intervening region. This study demonstrates the first efficient implementation of the CRISPR/Cas9 technology in the olive fly, providing new opportunities towards the development of novel genetic tools for its control. |
| Γλώσσα: | Αγγλικά |
| Σελίδες: | 9 |
| DOI: | 10.1002/arch.21667 |
| EISSN: | 1520-6327 |
| Θεματική κατηγορία: | [EL] Άλλες Επιστήμες Μηχανικού και Τεχνολογίες[EN] Other Engineering and Technologies [EL] Γεωπονική Βιοτεχνολογία[EN] Agricultural Biotechnology |
| Λέξεις-κλειδιά: | CRISPR/Cas9 ribonucleoprotein complexes; genome engineering; insect pest control; Tephritidae; white gene |
| Κάτοχος πνευματικών δικαιωμάτων: | © 2020 Wiley Periodicals, Inc. |
| Ηλεκτρονική διεύθυνση του τεκμηρίου στον εκδότη: | https://onlinelibrary.wiley.com/doi/abs/10.1002/arch.21667 |
| Ηλεκτρονική διεύθυνση περιοδικού: | https://onlinelibrary.wiley.com/journal/15206327 |
| Τίτλος πηγής δημοσίευσης: | Archives of Insect Biochemistry and Physiology |
| Τεύχος: | 2 |
| Τόμος: | 104 |
| Σελίδες τεκμηρίου (στην πηγή): | Article no e21667 |
| Σημειώσεις: | The authors would like to thank Kostas Bourtzis, Carlos Caceres, and Ahmad Sohel (FAO‐IAEA, Seibersdorf, Vienna, Austria) for providing olive fly pupae. Konstantina T. Tsoumani was supported through a postdoctoral scholarship from the Hellenic State Scholarship Foundation (IKY). This study is co‐financed by Greece and the European Union (European Social Fund) through the Operational Programme «Human Resources Development, Education and Lifelong Learning» in the context of the project “Reinforcement of Postdoctoral Researchers” (MIS‐5001552), implemented by the IKY. Angela Meccariello and Pasquale Primo were supported through PhD fellowships (2014‐2017 and 2018‐2021, respectively) of the Department of Biology, Federico II of Naples (Italy). |
| Εμφανίζεται στις συλλογές: | Μεταδιδακτορικοί ερευνητές |
Αρχεία σε αυτό το τεκμήριο:
| Αρχείο | Περιγραφή | Σελίδες | Μέγεθος | Μορφότυπος | Έκδοση | Άδεια | |
|---|---|---|---|---|---|---|---|
| 2_Meccariello, Tsoumani et al 2020.pdf | 963.2 kB | Adobe PDF | Δημοσιευμένη/του Εκδότη |  | Δείτε/ανοίξτε |