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https://hdl.handle.net/123456789/833| Τύπος: | Αναρτημένη ανακοίνωση (poster) |
| Τίτλος: | Dabigatran inhibits the activation of endothelial progenitor cells induced by thrombin |
| Συγγραφέας: | [EL] Παπαδάκη, Στυλιανή[EN] Papadaki, Styliani [EL] Σιδηροπούλου, Σοφία[EN] Sidiropoulou, Sofia [EL] Μοσχονάς, Ηρακλής[EN] Moschonas, Iraklis [EL] Τσελέπης, Αλέξανδρος[EN] Tselepis, Alexandros |
| Ημερομηνία: | 31/08/2019 |
| Περίληψη: | Introduction: Thrombin is a coagulation serine protease, which also activates various cell types, including endothelial cells. Late-outgrowth endothelial cells (OECs) are a type of endothelial progenitor cells that contribute to endothelial regeneration and angiogenesis. Dabigatran is a direct oral anticoagulant that inhibits thrombin’s action and is widely used in everyday clinical practice. Purpose: We investigated the effect of dabigatran on thrombin-induced activation of OECs, using the membrane expression of adhesion molecule ICAM-1 as activation marker. Control experiments were conducted in mature human umbilical vein endothelial cells (HUVECs). Methods: CD34+ cells were isolated from cord blood mononuclear cells, using human CD34 Microbead Kit and appropriately cultured for OEC formation. HUVECs were purchased from Lonza. Confluent OECs and HUVECs were incubated with 10 or 20 μM dabigatran for 10 min, before activation with 8 U/mL thrombin, for 24 h. The effect of dabigatran on ICAM-1 expression (anti-CD54-PE) was evaluated in both cell types as % gated CD31+/CD54+ cells, using flow cytometry. Results: Thrombin induced ICAM-1 membrane expression by 33±15% on OECs and by 123±22% on HUVECs, compared with respective untreated cells (p<0.05 for both comparisons, from 6 different experiments). Dabigatran at 10 μM significantly inhibited ICAM-1 expression by 36±7% on OECs and 57±8% on HUVECs, as well as at 20 μM by 51±8% on OECs and 79±7% on HUVECs (p<0.05 for all comparisons, from 4 different experiments). Conclusions: We show for the first time that dabigatran at concentrations relevant to those existing in vivo after oral administration, inhibits the thrombin-induced membrane expression of the adhesion molecule ICAM-1 on OECs, a phenomenon that is also observed on mature endothelial cells. The significance of this effect regarding the pathophysiological role of OECs and mature endothelial cells at the clinical level remains to be established. |
| Γλώσσα: | Αγγλικά |
| Τόπος δημοσίευσης: | Paris, France |
| Σελίδες: | 1 |
| DOI: | 10.1093/eurheartj/ehz745.0346 |
| ISSN: | 0195-668X |
| Θεματική κατηγορία: | [EL] Άλλες Ιατρικές Επιστήμες[EN] Other Medical Sciences |
| Λέξεις-κλειδιά: | Anticoagulants |
| Κάτοχος πνευματικών δικαιωμάτων: | © The Author(s) 2019 |
| Όροι και προϋποθέσεις δικαιωμάτων: | Published on behalf of the European Society of Cardiology. For permissions, please email: journals.permissions@oup.com. |
| Διατίθεται ανοιχτά στην τοποθεσία: | https://www.researchgate.net/publication/336693866_Dabigatran_inhibits_the_activation_of_endothelial_progenitor_cells_induced_by_thrombin https://esc365.escardio.org/Congress/ESC-CONGRESS-2019/Poster-Session-4-Anticoagulant-therapy/197331-dabigatran-inhibits-the-activation-of-endothelial-progenitor-cells-induced-by-thrombin#abstract |
| Ηλεκτρονική διεύθυνση του τεκμηρίου στον εκδότη: | https://esc365.escardio.org/Congress/ESC-CONGRESS-2019/Poster-Session-4-Anticoagulant-therapy/197331-dabigatran-inhibits-the-activation-of-endothelial-progenitor-cells-induced-by-thrombin#abstract https://academic.oup.com/eurheartj/article-abstract/40/Supplement_1/ehz745.0346/5597734 |
| Ηλεκτρονική διεύθυνση περιοδικού: | https://academic.oup.com/eurheartj |
| Τίτλος πηγής δημοσίευσης: | European Heart Journal |
| Τεύχος: | Supplement 1 |
| Τόμος: | 40 |
| Σελίδες τεκμηρίου (στην πηγή): | Article no P3475 |
| Όνομα εκδήλωσης: | ESC Congress 2019 together with the World Congress of Cardiology |
| Τοποθεσία εκδήλωσης: | Paris, France |
| Ημ/νία έναρξης εκδήλωσης: | 31/08/2019 |
| Ημ/νία λήξης εκδήλωσης: | 04/09/2019 |
| Σημειώσεις: | We thank the Atherothrombosis Research Centre of the University of Ioannina for providing access to laboratory facilities. This research has been co-financed by the Operational Program “Human Resources Development, Education and Lifelong Learning” and is co-financed by the European Union (European Social Fund) and Greek national funds. |
| Εμφανίζεται στις συλλογές: | Ερευνητικές ομάδες |
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| Αρχείο | Περιγραφή | Σελίδες | Μέγεθος | Μορφότυπος | Έκδοση | Άδεια | |
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