1. Αποθετήριο Παραδοτέων Υποτρόφων Δράσεων ΕΣΠΑ Τριτοβάθμιας Εκπαίδευσης 2017-2023
2. Όλο το Αποθετήριο
3. Ερευνητικές ομάδες

Τύπος:	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Investigating transcriptomic induction of resistance and/or virulence in Listeria monocytogenes cells surviving sublethal antimicrobial exposure
Εναλλακτικός τίτλος:	Διερεύνηση μεταγραφικής επαγωγής αντίστασης ή/και μολυσματικότητας σε κύτταρα Listeria monocytogenes που επιβιώνουν από υποθανατηφόρο αντιμικροβιακή έκθεση
Συγγραφέας:	[EL] Κοκκώνη, Ελένη – Άννα[EN] Kokkoni, Eleni - Anna [EL] Ανδρίτσος, Νικόλαος[EN] Andritsos, Nikolaos [EL] Σακαρίκου, Χριστίνα[EN] Sakarikou, Christina [EL] Μιχαηλίδου, Σοφία[EN] Michailidou, Sofia [EL] Αργυρίου, Αναγνώστης[EN] Argiriou, Anagnostis
Επικεφαλής ερευνητικής ομάδας:	[EL] Γκιαούρης, Ευστάθιος[EN] Giaouris, Efstathios
Ημερομηνία:	08/10/2021
Περίληψη:	The potential transcriptomic induction of resistance and/or virulence in two L. monocytogenes strains belonging to the most frequent listeriosis-associated serovars (i.e., 1/2a and 4b), following their sublethal antimicrobial exposure, was studied through qPCR determination of the relative expres-sion of 10 selected related genes (i.e., groEL, hly, iap, inlA, inlB, lisK, mdrD, mdrL, prfA, and sigB). To induce sublethal stress, three common antimicrobials (i.e., benzalkonium chloride, thymol, and ampicillin) were individually applied for 2 h at 37 °C against stationary phase cells of each strain, each at a sublethal concentration. In general, the expression of most of the studied genes remained either stable or was significantly downregulated following the antimicrobial exposure, with some strain-specific differences to be yet recorded. Thymol provoked downregulation of most of the studied genes, significantly limiting the expression of 6/10 and 4/10 genes in the strains of ser. 1/2a and ser. 4b, respectively, including those coding for the master regulators of stress response and virulence (SigB and PrfA, respectively), in both strains. At the same time, the two genes coding for the invasion internalin proteins (InlA and InlB), with crucial role in the onset of L. monocytogenes pathogenesis, were both importantly upregulated in ser. 4b strain. The results obtained increase our knowledge of the stress physiology of L. monocytogenes under certain sublethal antimicrobial con-ditions that could be encountered within the food chain and in clinical settings, and may assist in better and more effective mitigation strategies.
Γλώσσα:	Αγγλικά
Σελίδες:	14
DOI:	10.3390/ foods10102382
ISSN:	2304-8158
Θεματική κατηγορία:	[EL] Άλλες Γεωπονικές επιστήμες[EN] Other agricultural sciences [EL] Επιστήμη και Τεχνολογία τροφίμων[EN] Food Science and Technology
Λέξεις-κλειδιά:	gene expression; Listeria monocytogenes; stress response; virulence; benzalkonium chloride; thymol; ampicillin; sublethal antimicrobial exposure; survival
Κάτοχος πνευματικών δικαιωμάτων:	© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Όροι και προϋποθέσεις δικαιωμάτων:	This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Ηλεκτρονική διεύθυνση του τεκμηρίου στον εκδότη:	https://www.mdpi.com/2304-8158/10/10/2382
Ηλεκτρονική διεύθυνση περιοδικού:	https://www.mdpi.com/journal/foods
Τίτλος πηγής δημοσίευσης:	Foods
Τεύχος:	10
Τόμος:	10
Σελίδες τεκμηρίου (στην πηγή):	Article no 2382
Σημειώσεις:	This article belongs to the Special Issue Foodborne Pathogens and Food Safety This research was funded in the context of the project “Transcriptional determination of combinatory induction of resistance (stress adaptation) and virulence in Listeria monocytogenes cells as a consequence of their exposure to antimicrobial compounds” (MIS 5049514) under the call for proposals “Supporting researchers with an emphasis on new researchers” (EDULLL 103). The project is co-financed by Greece and the European Union (European Social Fund- ESF) by the Operational Programme Human Resources Development, Education and Lifelong Learning 2014-2020.
Εμφανίζεται στις συλλογές:	Ερευνητικές ομάδες