1. Αποθετήριο Παραδοτέων Υποτρόφων Δράσεων ΕΣΠΑ Τριτοβάθμιας Εκπαίδευσης 2017-2023
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Τύπος:	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Genotype- and tissue-specific metabolic networks and hub genes involved in water-induced distinct sweet cherry fruit cracking phenotypes
Συγγραφέας:	[EL] Μιχαηλίδης, Μιχαήλ[EN] Michailidis, Michail [EL] Καραγιάννης, Ευάγγελος[EN] Karagiannis, Evangelos [EL] Μπαζάκος, Χρήστος[EN] Bazakos, Christos [EL] Τάνου, Γεωργία[EN] Tanou, Georgia [EL] Γανόπουλος, Ιωάννης[EN] Ganopoulos, Ioannis [EL] Μολασιώτης, Αθανάσιος[EN] Molassiotis, Athanasios
Ημερομηνία:	28/09/2021
Περίληψη:	Sweet cherry fruit cracking is a complex physiological disorder that causes significant economic losses. Despite many years of research there is a lack of understanding of the mechanisms involved in cracking. Here, skin and flesh tissue from the cracking susceptible ’Early Bigi’ and the cracking tolerant ‘Regina’ cultivars were sampled prior and just after water dipping treatment to identify water-affected metabolic networks that putatively involved in fruit cracking. Primary metabolites, most strongly those involved in sugars and amino acid metabolism, such as glucose and asparagine, shifted in ’Early Bigi’ compared with ‘Regina’ tissues following water exposure. Comparisons between cultivars, tissues and dipping points identified significant differentially expressed genes. Particularly, genes related to abscisic acid, ethylene biosynthesis, pectin metabolism, expansins and aquaporins were altered in water-exposed tissues. To further characterize the role of these genes in cracking, their single nucleotide variants of the coding regions was studied in another eight sweet cherry cultivars, which differ in their sensitivity to cracking, revealing a strong link mainly between pectin metabolism-related genes and cracking phenotypes. Integrated metabolomic and transcriptomic profiling uncovered genotypic- and tissue specific metabolic pathways, including tricarboxylic acid cycle, cell enlargement, lipid and ethanol biosynthesis, and plant defense that putatively are involved in fruit cracking. Based on these results, a model which describes the skin and flesh metabolic reprogramming during water-induced fruit cracking in the susceptible ’Early Bigi’ cultivar is presented. Τhis study can help to explore novel candidate genes and metabolic pathways for cracking tolerance in sweet cherry
Γλώσσα:	Αγγλικά
Σελίδες:	15
DOI:	https://doi.org/10.1016/j.csbj.2021.09.030
ISSN:	2001-0370
Θεματική κατηγορία:	[EL] Φυτοκομία[EN] Horticulture
Λέξεις-κλειδιά:	Abscisic acid; Ethylene; Expansins; Flesh and skin tissues; Fruit cracking; Metabolomics; pectin; Sweet cherry; transcriptomics; Water dipping
Κάτοχος πνευματικών δικαιωμάτων:	© 2021 The Author(s). Published by Elsevier B.V on behalf of Research Network of Computational and Structural Biotechnology.
Όροι και προϋποθέσεις δικαιωμάτων:	This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Ηλεκτρονική διεύθυνση του τεκμηρίου στον εκδότη:	https://www.sciencedirect.com/science/article/pii/S2001037021004153
Ηλεκτρονική διεύθυνση περιοδικού:	https://www.sciencedirect.com/journal/computational-and-structural-biotechnology-journal
Τίτλος πηγής δημοσίευσης:	Computational and Structural Biotechnology Journal
Τεύχος:	Volume 19
Σελίδες τεκμηρίου (στην πηγή):	5406-5420
Σημειώσεις:	This research is co-financed by Greece and the European Union (European Social Fund- ESF) through the Operational Programme ‘Support for Researchers with Emphasis on Young Researchers’ in the context of the project ‘EDBM103-Part B, 2020-20220 (MIS: 5047887).
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