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Τύπος: Αναρτημένη ανακοίνωση (poster)
Τίτλος: Characterization of hub genes involved in sweet cherry fruit cracking
Συγγραφέας: [EL] Μιχαηλίδης, Μιχαήλ[EN] Michailidis, Michailsemantics logo
[EL] Καραγιάννης, Ευάγγελος[EN] Karagiannis, Evangelossemantics logo
[EL] Μπαζάκος, Χρήστος[EN] Bazakos, Christossemantics logo
[EL] Τάνου, Γεωργία[EN] Tanou, Georgiasemantics logo
[EL] Γανόπουλος, Ιωάννης[EN] Ganopoulos, Ioannissemantics logo
[EL] Μολασιώτης, Αθανάσιος[EN] Molassiotis, Athanasiossemantics logo
Ημερομηνία: 10/11/2021
Περίληψη: Sweet cherry fruit cracking is a complex physiological disorder that causes significant economic losses. Despite many years of research there is a lack of understanding of the mechanisms involved in cracking. Here, skin and flesh tissue from the cracking susceptible 'Early Bigi’ and the cracking tolerant ‘Regina’ cultivars were sampled prior and just after water dipping treatment to identify water-affected metabolic networks that putatively involved in fruit cracking. Primary metabolites, most strongly those involved in sugars and amino acid metabolism, such as glucose and asparagine, shifted in 'Early Bigi’ compared with ‘Regina’ tissues following water exposure. Comparisons between cultivars, tissues and dipping points identified significant differentially expressed genes. Particularly, genes related to abscisic acid, ethylene biosynthesis, pectin metabolism, expansins and aquaporins were altered in water-exposed tissues. To further characterize the role of these genes in cracking, their single nucleotide variants of the coding regions was studied in another eight sweet cherry cultivars, which differ in their sensitivity to cracking, revealing a strong link mainly between pectin metabolism-related genes and crackingphenotypes. Integrated metabolomic and transcriptomic profiling uncovered genotypic- and tissue-specific metabolic pathways, including tricarboxylic acid cycle, cell enlargement, lipid and ethanol biosynthesis, and plant defense that putatively are involved in fruit cracking. Based on these results, a model which describes the skin and flesh metabolic reprogramming during waterinduced fruit cracking in the susceptible 'Early Bigi’ cultivar is presented. Τhis study can help to explore novel candidate genes and metabolic pathways for cracking tolerance in sweet cherry.
Γλώσσα: Αγγλικά
Τόπος δημοσίευσης: France
Σελίδες: 1
Θεματική κατηγορία: [EL] Φυτοκομία[EN] Horticulturesemantics logo
Κάτοχος πνευματικών δικαιωμάτων: © The Author(s) 2021.
Όνομα εκδήλωσης: 1st International Symposium on Reproductive Biology of Fruit Tree Species, 8-10 November 2021, France.
Τοποθεσία εκδήλωσης: France
Ημ/νία έναρξης εκδήλωσης: 08/11/2021
Ημ/νία λήξης εκδήλωσης: 12/11/2021
Σημειώσεις: Symposium site: https://www.ishs.org/symposium/388
This research is co-financed by Greece and the European Union (European Social Fund- ESF) through the Operational Programme ‘Support for Researchers with Emphasis on Young Researchers’ in the context of the project ‘EDBM103-Part B, 2020-2022’ (MIS: 5047887)
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