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Τύπος: Άρθρο σε επιστημονικό περιοδικό
Τίτλος: Evaluation of basidiomycetes wild strains grown in agro-industrial residues for their anti-tyrosinase and antioxidant potential and for the production of biocatalysts
Συγγραφέας: [EL] Ζέρβα, Αναστασία[EN] Zerva, Anastasiasemantics logo
[EL] Τσαφαντάκης, Νικόλαος[EN] Tsafantakis, Nikolaossemantics logo
[EL] Τόπακας, Ευάγγελος[EN] Topakas, Evangelossemantics logo
Ημερομηνία: 01/02/2021
Περίληψη: White-rot basidiomycetes are the only microorganisms with the ability to produce both hydrolytic (cellulases and hemicellulases) and oxidative (ligninolytic) enzymes for degrading cel- lulose/hemicellulose and lignin. In addition, they produce biologically active natural products with important application in cosmetic formulations, either as pure compounds or as standardized extracts. In the present work, three wild strains of Basidiomycetes fungi (Pleurotus citrinopileatus, Abortiporus biennis and Ganoderma resinaceum) from Greek habitats were grown in agro-industrial residues (oil mill wastewater, and corn cob) and evaluated for their anti-tyrosinase and antioxidant activity and for the production of biotechnologically relevant enzymes. P. citrinopileatus showed the most interesting tyrosinase inhibitory activity, while A. biennis showed the highest DPPH(2,2- diphenyl-1-picryl-hydrazyl) scavenging potential. Corn cobs were the most appropriate carbon source for maximizing the inhibitory effect of fungal biomasses on both activities, while the use of oil mill wastewater selectively increased the anti-tyrosinase potential of P. citrinopileatus culture filtrate. All strains were found to be preferential lignin degraders, similarly to most white-rot fungi. Bioinformatic analyses were performed on the proteome of the strains P. citrinopileatus and A. biennis, focusing on CAZymes with biotechnological relevance, and the results were compared with the enzyme activities of culture supernatants. Overall, all three strains showed strong production of oxidative enzymes for biomass conversion applications.
Γλώσσα: Αγγλικά
Σελίδες: 12
DOI: 10.3390/ fermentation7010019
Θεματική κατηγορία: [EL] Μηχανική, διεπιστημονική προσέγγιση[EN] Engineering, interdisciplinarysemantics logo
Λέξεις-κλειδιά: basidiomycetesnovel enzymesbiocatalystsanti-tyrosinase activityAntioxidant activitylaccasesLPMOs
Κάτοχος πνευματικών δικαιωμάτων: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Όροι και προϋποθέσεις δικαιωμάτων: This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
Ηλεκτρονική διεύθυνση του τεκμηρίου στον εκδότη: https://www.mdpi.com/2311-5637/7/1/19
Ηλεκτρονική διεύθυνση περιοδικού: https://www.mdpi.com/journal/fermentation
Τίτλος πηγής δημοσίευσης: Fermentation
Τεύχος: 1
Τόμος: 7
Σελίδες τεκμηρίου (στην πηγή): Article no 19
Σημειώσεις: This research is funded in the context of the project “Biotechnological exploitation of wild strains of Greek basidiomycetes for the production of bioactive small molecules and macromolecules with applications in cosmetic and biocatalysis industries—BIO2” (MIS 5049430) under the call for proposals “Researchers’ support with an emphasis on young researchers- 2nd Cycle”. The project is co-financed by Greece and the European Union (European Social Fund- ESF) by the Operational Programme Human Resources Development, Education and Lifelong Learning 2014–2020.
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