1. Αποθετήριο Παραδοτέων Υποτρόφων Δράσεων ΕΣΠΑ Τριτοβάθμιας Εκπαίδευσης 2017-2023
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3. Ερευνητικές ομάδες

Τύπος:	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Differential immunohistochemical expression of hTERT in lung cancer patients with and without idiopathic pulmonary fibrosis
Συγγραφέας:	[EL] Γομάτου, Γεωργία[EN] Gomatou, Georgia [EL] Μασαούτης, Χρήστος[EN] Masaoutis, Christos [EL] Βαμβακάρης, Ιωάννης[EN] Vamvakaris, Ioannis [EL] Κοττέας, Ηλίας[EN] Kotteas, Ilias [EL] Μπούρος, Ευάγγελος[EN] Bouros, Evangelos [EL] Τζίλας, Βασίλειος[EN] Tzilas, Vasilios [EL] Μπούρος, Δημοσθένης[EN] Bouros, Demosthenes
Ημερομηνία:	10/02/2022
Περίληψη:	Background: Human telomerase reverse transcriptase (hTERT) is the catalytic subunit of telomerase enzyme, which adds nucleotides to telomeres and counteracts their length shortening. The development of a telomere maintenance mechanism represents a hallmark of cancer. On the other hand, idiopathic pulmonary fibrosis (IPF) is associated with mutations in telomerase genes and shorter telomeres. IPF is frequently complicated with lung cancer. Aim: To investigate the expression of hTERT in lung cancer with co-existing IPF and to compare with lung cancer without fibrosis. Methods: Diagnostic lung cancerous biopsies were retrieved from 18 patients with lung cancer and concomitant IPF, as well as 18 age and gender matched controls with lung cancer without pulmonary fibrosis. The expression of hTERT was studied with immunohistochemistry. ImajeJ software was used to quantitate subcellular stain intensity. Immunohistochemical investigation of two senescence-associated markers, p16 and p21, was also performed in all 36 cases. Results: Both groups highly expressed hTERT, without significant difference (100% vs 95%, p = 0.521). Evaluation of p16 and p21 immunostaining revealed negative to minimal immunoreactivity in both groups. hTERT localization exhibited higher median nuclear intensity in the group of lung cancer with IPF (0.62 vs 0.45, p = 0.016), while cytoplasmic intensity did not differ significantly (0.17 vs 0.15, p = 0.463). Higher median nuclear intensity was also correlated with small cell lung cancer subtype in the whole study sample (0.69 vs 0.45, p = 0.09). Conclusion: hTERT is highly expressed in lung cancer with concomitant IPF, but with differential localization compared to lung cancer without IPF, implying differences in pathogenicity and requiring further investigation.
Γλώσσα:	Αγγλικά
Σελίδες:	8
DOI:	10.1016/j.pulmoe.2021.12.001
ISSN:	2531-0437
Θεματική κατηγορία:	[EL] Πνευμονολογία και Αναπνευστικές παθήσεις[EN] Pulmonary and Respiratory Medicine [EL] Ογκολογία[EN] Oncology
Λέξεις-κλειδιά:	Idiopathic Pulmonary Fibrosis; Lung cancer; Telomerase reverse transcriptase; Telomerase; Small cell lung cancer
Κάτοχος πνευματικών δικαιωμάτων:	© 2022 Sociedade Portuguesa de Pneumologia. Published by Elsevier España, S.L.U
Όροι και προϋποθέσεις δικαιωμάτων:	This is an open access article under the CC BYNC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Ηλεκτρονική διεύθυνση του τεκμηρίου στον εκδότη:	https://www.sciencedirect.com/science/article/pii/S2531043722000022
Ηλεκτρονική διεύθυνση περιοδικού:	https://www.sciencedirect.com/journal/pulmonology
Τίτλος πηγής δημοσίευσης:	Pulmonology
Αρ. Φύλλου:	(Feb.2024) In Press, Corrected Proof, Available online 10 February 2022
Σημειώσεις:	This research is co-financed by Greece and the European Union (European Social Fund- ESF) through the Operational Programme «Human Resources Development, Education and Lifelong Learning 2014-2020» in the context of the project “The role of telomeres and telomerase in IPF-associated lung cancer” (MIS 5047957).
Εμφανίζεται στις συλλογές:	Ερευνητικές ομάδες