1. Αποθετήριο Παραδοτέων Υποτρόφων Δράσεων ΕΣΠΑ Τριτοβάθμιας Εκπαίδευσης 2017-2023
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3. Μεταδιδακτορικοί ερευνητές

Τύπος:	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	An ATM–Chk2–INCENP pathway activates the abscission checkpoint
Συγγραφέας:	[EL] Πετσαλάκη, Ελένη[EN] Petsalaki, Eleni [EL] Ζάχος, Γεώργιος[EN] Zachos, George
Ημερομηνία:	23/11/2020
Περίληψη:	During cell division, in response to chromatin bridges, the chromosomal passenger complex (CPC) delays abscission to prevent chromosome breakage or tetraploidization. Here, we show that inhibition of ATM or Chk2 kinases impairs CPC localization to the midbody center, accelerates midbody resolution in normally segregating cells, and correlates with premature abscission and chromatin breakage in cytokinesis with trapped chromatin. In cultured human cells, ATM activates Chk2 at late midbodies. In turn, Chk2 phosphorylates human INCENP-Ser91 to promote INCENP binding to Mklp2 kinesin and CPC localization to the midbody center through Mklp2 association with Cep55. Expression of truncated Mklp2 that does not bind to Cep55 or nonphosphorylatable INCENP-Ser91A impairs CPC midbody localization and accelerates abscission. In contrast, expression of phosphomimetic INCENP-Ser91D or a chimeric INCENP protein that is targeted to the midbody center rescues the abscission delay in Chk2-deficient or ATM-deficient cells. Furthermore, the Mre11–Rad50–Nbs1 complex is required for ATM activation at the midbody in cytokinesis with chromatin bridges. These results identify an ATM–Chk2–INCENP pathway that imposes the abscission checkpoint by regulating CPC midbody localization
Γλώσσα:	Αγγλικά
Σελίδες:	30
DOI:	10.1083/jcb.202008029
EISSN:	1540-8140
Θεματική κατηγορία:	[EL] Βασική ιατρική, άλλοι τομείς[EN] Basic medicine, miscellaneous
Κάτοχος πνευματικών δικαιωμάτων:	© 2020 Petsalaki and Zachos
Όροι και προϋποθέσεις δικαιωμάτων:	This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/)
Ηλεκτρονική διεύθυνση του τεκμηρίου στον εκδότη:	https://rupress.org/jcb/article/220/2/e202008029/211635/An-ATM-Chk2-INCENP-pathway-activates-the
Ηλεκτρονική διεύθυνση περιοδικού:	https://rupress.org/jcb
Τίτλος πηγής δημοσίευσης:	Journal of Cell Biology (JCB)
Τεύχος:	2
Τόμος:	220
Σελίδες τεκμηρίου (στην πηγή):	Article no e202008029
Σημειώσεις:	Work in our laboratory was supported by Fondation Sante. E. ´ Petsalaki was supported by the State Scholarships Foundation (project “Reinforcement of Postdoctoral Researchers - 2nd Cycle” grant MIS-5033021), cofinanced by Greece and the European Union (European Social Fund) through the Operational Program Human Resources Development, Education and Lifelong Learning.
Εμφανίζεται στις συλλογές:	Μεταδιδακτορικοί ερευνητές