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https://hdl.handle.net/123456789/1671| Τύπος: | Άρθρο σε επιστημονικό περιοδικό |
| Τίτλος: | In vitro anti-biofilm activity of bacteriophage K (ATCC 19685-B1) and daptomycin against staphylococci |
| Συγγραφέας: | [EL] Πλώτα, Μαρία[EN] Plota, Maria [EL] Σαζακλή, Ελένη[EN] Sazakli, Eleni [EL] Γιορμέζης, Νικόλαος[EN] Giormezis, Nikolaos [EL] Γκάρτζιου, Φωτεινή[EN] Gkartziou, Foteini [EL] Κολονίτσιου, Φεβρονία[EN] Kolonitsiou, Fevronia [EL] Λεοτσινίδης, Μιχάλης[EN] Leotsinidis, Michael [EL] Αντιμησιάρη, Σοφία[EN] Antimisiaris, Sophia [EL] Σπηλιοπούλου - Σδούγκου, Ίρις - Βασιλική[EN] Spiliopoulou - Sdugou Iris |
| Ημερομηνία: | 31/08/2021 |
| Περίληψη: | The purpose of the present study was to investigate anti-staphylococcal activity of daptomycin and bacteriophage K, alone or in combination, against biofilm-producers and non-producers S. aureus and S. epidermidis strains, under biofilm forming and cells’ proliferation conditions. Daptomycin and bacteriophage K (ATCC 19685B1), in different concentrations, were tested against 10 Staphylococcus aureus and 10 S. epidermidis, characterized by phenotypes and genotypes. The quantitative microtiter plate (crystal violet, CV), methylthiazoltetrazolium (MTT), and growth curve (GC) assays were performed. No statistically significant difference was found between species, whereas daptomycin alone performed better using medium and high concentrations of the drug and bacteriophage K was more active against strains with higher susceptibility, by CV and MTT assays. Best results were achieved using both agents combined in high concentrations. Bacteriophage K was effective within 3.8 and 2.4 h, depending on the concentration used, by the GC assay. Combination of daptomycin with bacteriophage K was more effective against staphylococci, depending on the concentrations used and strains’ susceptibility. Further studies are needed to evaluate if this approach might be a choice for prevention or therapy of biofilm-associated infections |
| Γλώσσα: | Αγγλικά |
| Σελίδες: | 16 |
| DOI: | 10.3390/microorganisms9091853 |
| EISSN: | 2076-2607 |
| Θεματική κατηγορία: | [EL] Τεχνολογία ιατρικών εργαστηρίων[EN] Medical Laboratory Technology |
| Λέξεις-κλειδιά: | biofilm; staphylococci; Daptomycin; Bacteriophage; prevention; phage K |
| Κάτοχος πνευματικών δικαιωμάτων: | © 2021 by the authors. Licensee MDPI, Basel, Switzerland. |
| Όροι και προϋποθέσεις δικαιωμάτων: | This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). |
| Ηλεκτρονική διεύθυνση του τεκμηρίου στον εκδότη: | https://www.mdpi.com/2076-2607/9/9/1853 |
| Ηλεκτρονική διεύθυνση περιοδικού: | https://www.mdpi.com/journal/microorganisms |
| Τίτλος πηγής δημοσίευσης: | Microorganisms |
| Τεύχος: | 9 |
| Τόμος: | 9 |
| Σελίδες τεκμηρίου (στην πηγή): | Article no 1853 |
| Σημειώσεις: | This research was funded by the Research Committee of the University of Patras, Greece, grant number 39540000 awarded to I.S. and co-funded (including APC) by the following grants received by Institute of Chemical Engineering Sciences, FORTH/ICE-HT: (1) the Operational Program “Human Resources Development, Education and Lifelong Learning 2014–2020”, co-financed by Greece and the European Union (European Social Fund-ESF) through in the context of the subproject “Innovative lipid nanoparticles for treatment of staphylococcal biomembranes” (MIS 5049223), grant number 39540000 awarded to S.G.A and I.S, and (2) the Operational Program “Competitiveness, Entrepreneurship and Innovation” (NSRF 2014–2020), co-financed by Greece and the European Union (European Regional Development Fund) subproject “Preclinical development of INNOvative FORmulations of antibiotics for intraocular administration for the treatment/prevention of postoperative endophthalmtis-Innofor I” (MIS 5031792) which is implemented under the Special Service of the Operational Program Competitiveness Entrepreneurship and Innovation. |
| Εμφανίζεται στις συλλογές: | Ερευνητικές ομάδες |
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