1. Αποθετήριο Παραδοτέων Υποτρόφων Δράσεων ΕΣΠΑ Τριτοβάθμιας Εκπαίδευσης 2017-2023
2. Όλο το Αποθετήριο
3. Ερευνητικές ομάδες

Τύπος:	Ανακοίνωση σε συνέδριο
Τίτλος:	Evaluation of opposite type heterologous MAT genes transfer, in the filamentous fungi Neofusicoccum mediterraneum and Verticillium dahliae
Συγγραφέας:	[EL] Παλαβούζης, Σταύρος[EN] Palavouzis, Stavros [EL] Τριανταφυλλοπούλου, Αλεξάνδρα[EN] Triantafyllopoulou, Alexandra [EL] Παπλωματάς, Επαμεινώντας[EN] Paplomatas, Epaminondas [EL] Τζίμα, Αλίκη[EN] Tzima, Aliki
Ημερομηνία:	23/08/2021
Περίληψη:	Mating type genes are present in most filamentous fungi, even though teleomorphs for all species have not been recorded. Our study, tries to explore the effect of different growth conditions on the expression of MAT genes in Neofusicoccum mediterraneum. As such, selected isolates were grown in potato dextrose broth or in Water Agar supplemented with pine needles under a 12 h photoperiod, as well as in constant darkness. Mycelia and spores were collected at different time points and RNA extraction was performed, with the extracted product being used for cDNA synthesis. New primers for MAT gene expression were designed, while qPCR results are underway. The second part of the study involved the isolation and cloning in a selected pGEM-T vector, of the Botryosphaeria dothidea MAT1-1-1 and MAT1-2-1 mating genes including flanking regions. As a next step, the genes were amplified using newly designed primers with engineered restriction sites. Amplicons were excised and subsequently sub-cloned in appropriate binary vectors. The constructs were afterwards inserted into Agrobacterium tumefaciens and utilized for Agrobacterium-mediated transformation (ATMT) of Neofusicoccum mediterraneum. At the same time, transformation of a Verticillium dahliae tomato race 1 strain (70V) was performed as control. While the procedure was successful in regards to V. dahliae, transformed strains of N. mediterraneum could not be obtained. At present, a new transformation protocol, which utilizes a combination of protoplast and Agro transformation, is being evaluated.
Γλώσσα:	Αγγλικά
Τόπος δημοσίευσης:	Rome, Italy
Σελίδες:	57
Θεματική κατηγορία:	[EL] Γεωπονία, διεπιστημονική προσέγγιση[EN] Agriculture, interdisciplinary
Λέξεις-κλειδιά:	Anamorph; heterothallism; perithecia; pycnidia; sexual stage
Κάτοχος πνευματικών δικαιωμάτων:	© The Author(s) 2021
Ηλεκτρονική διεύθυνση του τεκμηρίου στον εκδότη:	Not available - online conference site doesn't exist anymore
Ηλεκτρονική διεύθυνση περιοδικού:	Not available - online conference site doesn't exist anymore
Τίτλος πηγής δημοσίευσης:	irc 2021 XV. international research conference proceedings
Σελίδες τεκμηρίου (στην πηγή):	57
Όνομα εκδήλωσης:	ICPPD018 2021: XV. International Conference on Phytopathology and Plant Disorders
Τοποθεσία εκδήλωσης:	Rome, Italy
Ημ/νία έναρξης εκδήλωσης:	23/08/2021
Ημ/νία λήξης εκδήλωσης:	24/08/2021
Σημειώσεις:	The site of the conference is not available anymore. It was an online conference during COVID with title "Congress : ICPPP031 2021: 15. International Conference on Phytopathology and Plant Pathology". The current conference site for this year is https://www.icpp2023.org/. This Research Project is co-financed by Greece and the European Union (European Social Fund) through the Operational Program “Human Resources Development, Education and Lifelong Learning 2014-2020” and the Program encoded EDBM103, titled “Support for researchers with an emphasis on young researchers-cycle B '”, in the context of the project “Risk evaluation of development of a resistant form of disease via transfer of mating type genes from the Botryosphaeria dothidea species to species of the genus Neofusicoccum” (MIS 5048471).
Εμφανίζεται στις συλλογές:	Ερευνητικές ομάδες