Παρακαλώ χρησιμοποιήστε αυτό το αναγνωριστικό για να παραπέμψετε ή να δημιουργήσετε σύνδεσμο προς αυτό το τεκμήριο:
https://hdl.handle.net/123456789/344| Τύπος: | Άρθρο σε επιστημονικό περιοδικό |
| Τίτλος: | Enhanced catalytic performance of Trichoderma reesei cellulase immobilized on magnetic hierarchical porous carbon nanoparticles |
| Συγγραφέας: | [EL] Παπαδόπουλου, Αθηνά[EN] Papadopoulou, Athena [EL] Ζαραφέτα, Δήμητρα[EN] Zarafeta, Dimitra [EL] Γαλανοπούλου, Αναστασία[EN] Galanopoulou, Anastasia [EL] Σταμάτης, Χαράλαμπος[EN] Stamatis, Haralambos |
| Ημερομηνία: | 23/09/2019 |
| Περίληψη: | Cellulase from Trichoderma reesei was immobilized by covalent or non-covalent binding onto magnetic hierarchical porous carbon (MHPC) nanomaterials. The immobilization yield and the enzyme activity were higher when covalent immobilization approach was followed. The covalent immobilization approach leads to higher immobilization yield (up to 96%) and enzyme activity (up to 1.35 U mg−1) compared to the non-covalent cellulase binding. The overall results showed that the thermal, storage and operational stability of the immobilized cellulase was considerably improved compared to the free enzyme. The immobilized cellulose catalyzed the hydrolysis of microcrystalline cellulose up to 6 consecutive successive reaction cycles, with a total operation time of 144 h at 50 °C. The half-life time of the immobilized enzyme in deep eutectic solvents-based media was up to threefold higher compared to the soluble enzyme. The increased pH and temperature tolerance of the immobilized cellulase, as well as the increased operational stability in aqueous and deep eutectic solvents-based media indicate that the use of MHPCs as immobilization nanosupport could expand the catalytic performance of cellulolytic enzymes in various reaction conditions. |
| Γλώσσα: | Αγγλικά |
| Σελίδες: | 9 |
| DOI: | 10.1007/s10930-019-09869-w |
| ISSN: | 1572-3887 |
| Θεματική κατηγορία: | [EL] Βιομηχανική Βιοτεχνολογία[EN] Industrial Biotechnology |
| Λέξεις-κλειδιά: | Cellulase; Cellulose; Immobilization; Magnetic nanomaterials; Deep eutectic solvents; Stabilization |
| Κάτοχος πνευματικών δικαιωμάτων: | Copyright © 2019, Springer Science Business Media, LLC, part of Springer Nature |
| Όροι και προϋποθέσεις δικαιωμάτων: | https://s100.copyright.com/AppDispatchServlet?title=Enhanced%20Catalytic%20Performance%20of%20Trichoderma%20reesei%20Cellulase%20Immobilized%20on%20Magnetic%20Hierarchical%20Porous%20Carbon%20Nanoparticles&author=Athena%20Papadopoulou%20et%20al&contentID=10.1007%2Fs10930-019-09869-w©right=Springer%20Science%2BBusiness%20Media%2C%20LLC%2C%20part%20of%20Springer%20Nature&publication=1572-3887&publicationDate=2019-09-23&publisherName=SpringerNature&orderBeanReset=true |
| Ηλεκτρονική διεύθυνση του τεκμηρίου στον εκδότη: | https://link.springer.com/article/10.1007/s10930-019-09869-w#citeas |
| Ηλεκτρονική διεύθυνση περιοδικού: | https://www.springer.com/journal/10930 |
| Τίτλος πηγής δημοσίευσης: | The Protein Journal |
| Τεύχος: | 6 |
| Τόμος: | 38 |
| Σελίδες τεκμηρίου (στην πηγή): | 640 - 648 |
| Σημειώσεις: | We acknowledge support of this work by the project MIS 5005434 which has been co-financed by the Operational Program “Human Resources Development, Education and Lifelong Learning” and is co-financed by the European Union (European Social Fund) and Greek national funds. |
| Εμφανίζεται στις συλλογές: | Ερευνητικές ομάδες |
Αρχεία σε αυτό το τεκμήριο:
Το πλήρες κείμενο αυτού του τεκμηρίου δεν διατίθεται προς το παρόν από το αποθετήριο