1. Αποθετήριο Παραδοτέων Υποτρόφων Δράσεων ΕΣΠΑ Τριτοβάθμιας Εκπαίδευσης 2017-2023
2. Όλο το Αποθετήριο
3. Μεταδιδακτορικοί ερευνητές

Τύπος:	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Apoptotic markers in the midbrain of the human neonate after perinatal hypoxic/ischemic injury.
Συγγραφέας:	[EL] Παγίδα, Μαριάννα[EN] Pagida, Marianna [EL] Κωνσταντινίδου, Αναστασία[EN] Konstantinidou, Anastasia [EL] Χρυσάνθου-Πιτερού, Μαργαρίτα[EN] Chrysanthou-Piterou, Margarita [EL] Πατσούρης, Ευστράτιος[EN] Patsouris, Efstratios [EL] Παναγιωτακοπούλου, Μαρία[EN] Panayotacopoulou, Maria
Ημερομηνία:	10/12/2019
Περίληψη:	Our previous postmortem studies on neonates with neuropathological injury of perinatal hypoxia/ischemia (PHI) showed a dramatic reduction of tyrosine hydroxylase expression (dopamine synthesis enzyme) in substantia nigra (SN) neurons, with reduction of their cellular size. In order to investigate if the above observations represent an early stage of SN degeneration, we immunohistochemically studied the expression of cleaved caspase-3 (CCP3), apoptosis inducing factor (AIF), and DNA fragmentation by using terminal deoxynucleotidyltransferase-mediated dUTP-biotin 3′-end-labeling (TUNEL) technique in the SN of 22 autopsied neonates (corrected age ranging from 34 to 46.5 gestational weeks), in relation to the severity/duration of PHI injury, as estimated by neuropathological criteria. No CCP3-immunoreactive neurons and a limited number of apoptotic TUNEL-positive neurons with pyknotic characteristics were found in the SN. Nuclear AIF staining was revealed only in few SN neurons, indicating the presence of early signs of AIF-mediated degeneration. By contrast, motor neurons of the oculomotor nucleus showed higher cytoplasmic AIF expression and nuclear translocation, possibly attributed to the combined effect of developmental processes and increased oxidative stress induced by antemortem and postmortem factors. Our study indicates the activation of AIF, but not CCP3, in the SN and oculomotor nucleus of the human neonate in the developmentally critical perinatal period.
Γλώσσα:	Αγγλικά
Σελίδες:	16
DOI:	10.1093/jnen/nlz114
ISSN:	0022-3069
Θεματική κατηγορία:	[EL] Νευροεπιστήμες[EN] Neurosciences
Λέξεις-κλειδιά:	Apoptosis inducing factor (AIF); Cleaved caspase-3 (CCP3); DNA fragmentation; Human substantia nigra; Oculomotor nucleus; Perinatal hypoxia/ischemia; Perinatal infection
Κάτοχος πνευματικών δικαιωμάτων:	© 2019 American Association of Neuropathologists, Inc. All rights reserved.
Διατίθεται ανοιχτά στην τοποθεσία:	https://academic.oup.com/jnen/article/79/1/86/5653101
Ηλεκτρονική διεύθυνση του τεκμηρίου στον εκδότη:	https://academic.oup.com/jnen/article/79/1/86/5653101
Ηλεκτρονική διεύθυνση περιοδικού:	https://academic.oup.com/jnen
Τίτλος πηγής δημοσίευσης:	Journal of Neuropathology & Experimental Neurology
Τεύχος:	1
Τόμος:	79
Σελίδες τεκμηρίου (στην πηγή):	86-101
Σημειώσεις:	This work was supported by the program “Supporting Postdoctoral Researchers” (MIS: 5001552) from the State Scholarships Foundation, cofinanced by the Greek State and European Social Fund (fellowship to MPag). Fetal and perinatal pathology diagnostics and research are supported by REA Maternity Clinic, Athens, Greece (grants to AK, Project 11191/SARG, NKUA).
Εμφανίζεται στις συλλογές:	Μεταδιδακτορικοί ερευνητές