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Τύπος: Άρθρο σε επιστημονικό περιοδικό
Τίτλος: Cross-species testing of nuclear markers in Pelophylax water frogs in Greece and examination of their power to detect genetic admixture
Συγγραφέας: [EL] Σαγώνας, Κωνσταντίνος[EN] Sagonas, Kostassemantics logo
[EL] Καραμέτα, Εμμανουέλα[EN] Karameta, Emmanouelasemantics logo
[EL] Κοτσακιόζη, Παναγιώτα[EN] Kotsakiozi, Panagiotasemantics logo
[EL] Πουλακάκης, Νικόλαος[EN] Poulakakis, Nikolaossemantics logo
Ημερομηνία: 12/06/2020
Περίληψη: The genus Pelophylax has been considered a model organism for understanding hybridization. Despite being the most diverse within the Western Palearctic group of Pelophylax, the ridibundus/bedriagae lineage that includes six species, remains largely understudied, revealing many knowledge gaps in regards to their evolution and conservation. Using genetic data from populations among species inhabiting contact zones could prove vital in filling these gaps. We tested 17 microsatellite markers for cross-species amplification in mainland Pelophylax species distributed in southern Balkans and evaluated their power to successfully detect population/species structure. Importantly, we examined their potential for identifying hybrids and backcrosses between known hybridized species. We detected 12 highly polymorphic loci that crossamplified all species that showed no significant Linkage Disequilibrium and were able to discriminate among species and between parental and hybrids. We suggest their future use in genetic studies for the genus Pelophylax in Greece, including the identification of contact zones.
Γλώσσα: Αγγλικά
Σελίδες: 7
DOI: 10.1163/15685381-20191229
ISSN: 0173-5373
Θεματική κατηγορία: [EL] Φυσικές Επιστήμες[EN] Natural Sciencessemantics logo
Λέξεις-κλειδιά: amphibianshybridizationmicrosatellitesprimersspecies differentiation
Κάτοχος πνευματικών δικαιωμάτων: © Koninklijke Brill NV, Leiden, 2019.
Διατίθεται ανοιχτά στην τοποθεσία: https://brill.com/view/journals/amre/41/2/article-p253_7.xml
Ηλεκτρονική διεύθυνση του τεκμηρίου στον εκδότη: https://brill.com/view/journals/amre/41/2/article-p253_7.xml
Ηλεκτρονική διεύθυνση περιοδικού: https://brill.com/view/journals/amre/amre-overview.xml
Τίτλος πηγής δημοσίευσης: Amphibia-Reptilia
Τεύχος: 2
Τόμος: 41
Σελίδες τεκμηρίου (στην πηγή): 253-259
Σημειώσεις: Authors are grateful to Dr. Antoniou A. (Hellenic Centre for Marine Research; HCMR) for carrying out the microsatellite genotyping and for helping with the initial selection of microsatellite markers. We also thank two anonymous reviewers for valuable comments that significantly improved the manuscript. This project has been co-financed by the Operational Program “Human Resources Development, Education and Lifelong Learning” and is co-financed by the European Union (European Social Fund) and Greek national funds awarded to NP, KS and PK (EDBM34; 10068).
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