1. Αποθετήριο Παραδοτέων Υποτρόφων Δράσεων ΕΣΠΑ Τριτοβάθμιας Εκπαίδευσης 2017-2023
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Τύπος:	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Unraveling the blood transcriptome after real-life exposure of Wistar-rats to PM2.5, PM1 and water-soluble metals in the ambient air
Συγγραφέας:	[EL] Φρυδάς, Ηλίας[EN] Frydas, Ilias S. I.S. [EL] Κερμενίδου, Μαριάνθη[EN] Kermenidou, Marianthi [EL] Τσαβέ, Όλγα[EN] Tsave, Olga [EL] Σαλίφογλου, Αθανάσιος[EN] Salifoglou, Athanasios [EL] Σαρηγιάννης, Δημοσθένης[EN] Sarigiannis, Dimosthenis
Ημερομηνία:	21/10/2020
Περίληψη:	Exposure to particulate matter (PM) is one of the most important environmental issues in Europe with major health impact. Various sizes of PM are suspended in the atmosphere and contributes to ambient air pollution. The current study aimed to explore the differential gene expression in blood, and the effect on the respective biological signaling pathways in Wistar rats, after exposure to PM2.5 and PM1 ambient air particles for an eightweek period. A control group was included with animals breathing non-filtered atmospheric air. In parallel, filtered PM2.5 and PM1 was collected in separate samplers. The results after whole genome microarray analysis showed 23 differentially expressed genes (DEGs) between control and PM2.5 group. In addition, pairwise comparison between control and PM1 group displayed 5635 DEGs linked to 69 biological pathways involved in inflammatory response, cell cycle and carcinogenicity. The smaller the size of the inhaled particles, the more gene alterations are triggered compared to non-filtered air group. More specifically, in inflammation signaling procedures differentially regulated gene expression was shown for interleukin-4 (IL-4), IL-7, IL-1, IL-5, IL-9, IL-6 and IL-2. We have identified that RASGFR1, TRIM65, TRIM33, PLEKHB1, CAR4, S100A8, S100A9, ALPL, NP4 and the PROK2 genes are potential targets for the development of adverse outcome pathways (AOPs) due to “real-life” exposure of Wistar rats. Particle measurements during the exposure period showed elevated concentrations of Fe, Mn and Zn in both PM1 and PM2.5 filter fractions, and of Cu in PM2.5. In addition, watersoluble concentration of metals showed significant differences between PM1 and PM2.5 fractions for V, Zn, As, Pb and Mn. In summary, in this study specific gene biomarkers of exposure to ambient air have been identified and heavy metals that are possibly linked to their altered regulation have been found. The results of this research will pave the way for the development of novel AOPs concerning the health effects of the environmental pollution.
Γλώσσα:	Αγγλικά
Σελίδες:	11
DOI:	10.1016/j.toxrep.2020.10.014
ISSN:	2214-7500
Θεματική κατηγορία:	[EL] Δημόσια/περιβαλλοντική υγεία και Υγεία της εργασίας[EN] Public Health, Environmental and Occupational Health [EL] Βιοχημεία και Μοριακή βιολογία[EN] Biochemistry and Molecular Biology
Λέξεις-κλειδιά:	PM1; PM2.5; Microarrays; Exposure; Real-life; AOPs
Κάτοχος πνευματικών δικαιωμάτων:	© 2020 The Authors. Published by Elsevier B.V.
Όροι και προϋποθέσεις δικαιωμάτων:	This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Διατίθεται ανοιχτά στην τοποθεσία:	https://www.sciencedirect.com/science/article/pii/S2214750020304224
Ηλεκτρονική διεύθυνση του τεκμηρίου στον εκδότη:	https://www.sciencedirect.com/science/article/pii/S2214750020304224
Ηλεκτρονική διεύθυνση περιοδικού:	https://www.sciencedirect.com/journal/toxicology-reports
Τίτλος πηγής δημοσίευσης:	Toxicology Reports
Τόμος:	7
Σελίδες τεκμηρίου (στην πηγή):	1469-1479
Σημειώσεις:	This work was supported by the Operation Programme (EDBM34) in Human Resources Development, Education and Learning Co-funded by Greece and the European Union. Number of Scholarship: 95382. The authors gratefully acknowledge the financial support of the European Unions’ Horizon 2020 research and innovation Programmes under grant agreement No 733032 HBM4EU (www.HBM4EU.eu) and No 690105 ICARUS (www.icarus2020.eu).
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