1. Αποθετήριο Παραδοτέων Υποτρόφων Δράσεων ΕΣΠΑ Τριτοβάθμιας Εκπαίδευσης 2017-2023
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3. Ερευνητικές ομάδες

Τύπος:	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	miR-16-5p promotes erythroid maturation of erythroleukemia cells by regulating ribosome biogenesis
Συγγραφέας:	[EL] Παπαγιαννόπουλος, Χρήστος[EN] Papagiannopoulos, Christos [EL] Θεοδωρούλα, Νικολέτα[EN] Theodoroula, Nikoleta [EL] Βιζιριανάκης, Ιωάννης[EN] Vizirianakis, Ioannis
Ημερομηνία:	09/02/2021
Περίληψη:	miRNAs constitute a class of non-coding RNA that act as powerful epigenetic regulators in animal and plant cells. In order to identify putative tumor-suppressor miRNAs we profiled the expression of various miRNAs during differentiation of erythroleukemia cells. RNA was purified before and after differentiation induction and subjected to quantitative RT-PCR. The majority of the miRNAs tested were found upregulated in differentiated cells with miR-16-5p showing the most significant increase. Functional studies using gain- and loss-of-function constructs proposed that miR-16-5p has a role in promoting the erythroid differentiation program of murine erythroleukemia (MEL) cells. In order to identify the underlying mechanism of action, we utilized bioinformatic in-silico platforms that incorporate predictions for the genes targeted by miR-16-5p. Interestingly, ribosome constituents, as well as ribosome biogenesis factors, were overrepresented among the miR-16-5p predicted gene targets. Accordingly, biochemical experiments showed that, indeed, miR-16-5p could modulate the levels of independent ribosomal proteins, and the overall ribosomal levels in cultured cells. In conclusion, miR-16-5p is identified as a differentiation-promoting agent in erythroleukemia cells, demonstrating antiproliferative activity, likely as a result of its ability to target the ribosomal machinery and restore any imbalanced activity imposed by the malignancy and the blockade of differentiation.
Γλώσσα:	Αγγλικά
Σελίδες:	14
DOI:	10.3390/ph14020137
EISSN:	1424-8247
Θεματική κατηγορία:	[EL] Φαρμακολογία και Φαρμακευτική[EN] Pharmacology and Pharmacy
Λέξεις-κλειδιά:	miRNA therapeutics; miR-16-5p; erythroleukemia; erythroid differentiation; cancer; ribosomes
Κάτοχος πνευματικών δικαιωμάτων:	© 2021 by the authors. Licensee MDPI
Όροι και προϋποθέσεις δικαιωμάτων:	This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
Διατίθεται ανοιχτά στην τοποθεσία:	https://www.mdpi.com/1424-8247/14/2/137/htm
Ηλεκτρονική διεύθυνση του τεκμηρίου στον εκδότη:	https://www.mdpi.com/1424-8247/14/2/137
Ηλεκτρονική διεύθυνση περιοδικού:	https://www.mdpi.com/journal/pharmaceuticals
Τίτλος πηγής δημοσίευσης:	Pharmaceuticals
Τεύχος:	2
Τόμος:	14
Σελίδες τεκμηρίου (στην πηγή):	Article no 137
Σημειώσεις:	This research is co-financed by Greece and the European Union (European Social Fund—ESF) through the Operational Programme “Human Resources Development, Education and Lifelong Learning 2014–2020” in the context of the project “Investigation of the molecular function of miR-16-5p in ribosome biogenesis and its potential pharmacological exploitation in cancer” (MIS 5047886). (This article belongs to the Special Issue siRNA Therapeutics: From Bench Lab to Clinics)
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