1. Αποθετήριο Παραδοτέων Υποτρόφων Δράσεων ΕΣΠΑ Τριτοβάθμιας Εκπαίδευσης 2017-2023
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3. Ερευνητικές ομάδες

Τύπος:	Ανακοίνωση σε συνέδριο
Τίτλος:	Placental expression of neurokinin B and its receptor NK3R is increased in women with polycystic ovary syndrome: results of a preliminary study
Συγγραφέας:	[EL] Μαρκαντές, Γεώργιος[EN] Markantes, Georgios [EL] Μαρκάτος, Φώτιος[EN] Markatos, Fotios [EL] Γεωργόπουλος, Νεοκλής[EN] Georgopoulos, Neoklis
Ημερομηνία:	23/05/2021
Περίληψη:	Background: Women with polycystic ovary syndrome (PCOS) are at increased risk of pregnancy complications and poor pregnancy outcomes. Defective placentation is among the proposed mechanisms involved. Altered neurokinin B (NKB) placental expression has been associated with several conditions characterized by placental dysfunction, such as pre-eclampsia and intra-uterine growth retardation. However, the expression of NKB and its receptors has not been studied in placental tissue of women with PCOS. Objective: To compare the placental mRNA expression of NKB and its receptors NK1R, NK2R and NK3R in women with PCOS and controls. Methods: This was a single-center, prospective, case-control study. Women with PCOS according to the Rotterdam criteria (cases) and healthy pregnant women (controls) were enrolled at first prenatal visit and followed until delivery. Only women with spontaneous conception and singleton, uncomplicated, term pregnancies (10 PCOS and 10 controls) were included in the final analysis. All participants provided informed consent. At delivery, placental specimens were collected and immediately submerged in RNAlater solution. Samples were stored at -20oC until analysis. The mRNA expression of NKB, NK1R, NK2R and NK3R was quantified by real-time CR (RT-PCR). The relative mRNA expression was estimated by the ΔΔCT method, using β-actin as reference (housekeeping gene). Statistical analysis was performed using SPSS 25.0, and the level of statistical significance was set at 0.05 (two-sided). Results: The placental mRNA expression of NKB and NK3R was significantly higher in PCOS women versus controls (2.4-fold, p<0.05 for NKB and 7-fold, p<0.05 for NK3R). No significant alterations were observed in the mRNA expression of NK1R and NK2R between the two groups. There was no statistically significant difference regarding age, BMI, caesarian section frequency, offspring sex and birth weight between women with PCOS and controls. The placental expression of NKB and its receptors was correlated neither with maternal age and BMI, nor with offspring birth weight. Conclusions: The present study is the first to demonstrate increased placental expression of NKB and its receptor NK3R in women with PCOS. These findings support a potential role for NKB as a mediator of placental alterations characterizing PCOS. Expanding the number of participants is the necessary next step, in order to corroborate these preliminary findings. Furthermore, correlations between the placental expression of NKB, NK1R, NK2R, NK3R and PCOS phenotype, maternal sex steroids, glucose and insulin levels should be sought.
Γλώσσα:	Αγγλικά
Τόπος δημοσίευσης:	Virtual
Σελίδες:	13
DOI:	DOI: 10.1530/endoabs.73.YI10
EISSN:	1479-6848
Θεματική κατηγορία:	[EL] Επιστήμες υγείας, γενικά[EN] Health sciences, general
Λέξεις-κλειδιά:	polycystic ovary syndrome (PCOS); placenta; neurokinin B (NKB); NK3R
Κάτοχος πνευματικών δικαιωμάτων:	European Society of Endocrinology
Σχετίζεται με:	Placental expression of neurokinin B and its receptor NK3R is increased in women with polycystic ovary syndrome: results of a preliminary study
Ηλεκτρονική διεύθυνση του τεκμηρίου στον εκδότη:	https://www.endocrine-abstracts.org/ea/0073/ea0073yi10
Ηλεκτρονική διεύθυνση περιοδικού:	https://www.endocrine-abstracts.org/
Τίτλος πηγής δημοσίευσης:	Endocrine Abstracts
Τόμος:	73
Σελίδες τεκμηρίου (στην πηγή):	Article no YI10
Όνομα εκδήλωσης:	23rd European Congress of Endocrinology
Τοποθεσία εκδήλωσης:	Virtual Congress
Ημ/νία έναρξης εκδήλωσης:	22/05/2021
Ημ/νία λήξης εκδήλωσης:	26/05/2021
Σημειώσεις:	This research is co-financed by Greece and the European Union (European Social Fund - ESF) through the Operational Programme “Human Resources Development, Education and Lifelong Learning 2014-2020” in the context of the project “Exploring the developmental theory for polycystic ovary syndrome: the role of alterations in placental gene expression and in fetal DNA methylation (MIS: 5047128).”
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