1. Αποθετήριο Παραδοτέων Υποτρόφων Δράσεων ΕΣΠΑ Τριτοβάθμιας Εκπαίδευσης 2017-2023
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3. Ερευνητικές ομάδες

Τύπος:	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Effect of artemisinin-loaded mesoporous cerium-doped calcium silicate nanopowder on cell proliferation of human periodontal ligament fibroblasts
Συγγραφέας:	[EL] Τσαμεσίδης, Ιωάννης[EN] Tsamesidis, Ioannis [EL] Γκιλιόπουλος, Δημήτριος[EN] Giliopoulos, Dimitrios [EL] Πουρουτζίδου, Γεωργία[EN] Pouroutzidou, Georgia [EL] Λυμπεράκη, Ευγενία[EN] Lymperaki, Evgenia [EL] Παπούλια, Χρυσάνθη[EN] Papoulia, Chrysanthi [EN] Reybier, Karine [FR] Pério, Pierre [EL] Παρασκευόπουλος, Κωνσταντίνος[EN] Paraskevopoulos, Konstantinos [EL] Κοντονασάκη, Ελεάνα[EN] Kontonasaki, Eleana [EL] Θεοχαρίδου, Άννα[EN] Theocharidou, Anna
Ημερομηνία:	26/08/2021
Περίληψη:	Abstract Ion doping has rendered mesoporous structures important materials in the field of tissue engineering, as apart from drug carriers, they can additionally serve as regenerative materials. The purpose of the present study was the synthesis, characterization and evaluation of the effect of artemisinin (ART)-loaded cerium-doped mesoporous calcium silicate nanopowders (NPs) on the hemocompatibility and cell proliferation of human periodontal ligament fibroblasts (hPDLFs). Mesoporous NPs were synthesized in a basic environment via a surfactant assisted cooperative self-assembly process and were characterized using Scanning Electron Microscopy (SEM), X-ray Fluorescence Spectroscopy (XRF), Fourier Transform Infrared Spectroscopy (FT-IR), X-ray Diffraction Analysis (XRD) and N2 Porosimetry. The loading capacity of NPs was evaluated using Ultrahigh Performance Liquid Chromatography/High resolution Mass Spectrometry (UHPLC/HRMS). Their biocompatibility was evaluated with the MTT assay, and the analysis of reactive oxygen species was performed using the cell-permeable ROS-sensitive probe 2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA). The synthesized NPs presented a mesoporous structure with a surface area ranging from 1312 m2/g for undoped silica to 495 m2/g for the Ce-doped NPs, excellent bioactivity after a 1-day immersion in c-SBF, hemocompatibility and a high loading capacity (around 80%). They presented ROS scavenging properties, and both the unloaded and ART-loaded NPs significantly promoted cell proliferation even at high concentrations of NPs (125 μg/mL). The ART-loaded Ce-doped NPs with the highest amount of cerium slightly restricted cell proliferation after 7 days of culture, but the difference was not significant compared with the control untreated cells.
Γλώσσα:	Αγγλικά
Σελίδες:	21
DOI:	10.3390/nano11092189
EISSN:	2079-4991
Θεματική κατηγορία:	[EL] Βιο- υλικά[EN] Biomaterials
Λέξεις-κλειδιά:	mesoporous nanopowders; cerium doping; artemisinin loading; hemocompatibility; human periodontal ligament fibroblasts
Κάτοχος πνευματικών δικαιωμάτων:	© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Όροι και προϋποθέσεις δικαιωμάτων:	This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
Ηλεκτρονική διεύθυνση του τεκμηρίου στον εκδότη:	https://www.mdpi.com/2079-4991/11/9/2189
Ηλεκτρονική διεύθυνση περιοδικού:	https://www.mdpi.com/journal/nanomaterials
Τίτλος πηγής δημοσίευσης:	Nanomaterials
Τεύχος:	9
Τόμος:	11
Σελίδες τεκμηρίου (στην πηγή):	Article no 2189
Σημειώσεις:	Related Poster: Tsamesidis1, D. Gkiliopoulos2, G. K. Pouroutzidou1, E. Lymperaki3, E. Likotrafiti4, J. Rhoades4, E. Kontonasaki5, K. M. Paraskevoloulos1, A. Theocharidou. BIOACTIVE AND BIOCOMPATIBLE CERIUM DOPED MESOPOROUS NANOPARTICLES. 31 Annual Conference of the European Society for Biomaterials (ESB 2021) Poster #1194 This research is funded in the context of the project “Mesoporous nanocarriers with cerium ions (Ce-doped MSNs) for controlled release of active molecules aiming at osteogenic differentiation of human periodontal fibroblasts” (MIS: 5049566) under the call for proposals “Support for researchers with an emphasis on young researchers-second cycle” of the OP “Human Resources Development, Education and Lifelong Learning” (EDBM103). The project is co-financed by Greece and the European Union (European Social Fund-ESF) by the Operational Program Human Resources Development, Education and Lifelong Learning 2014–2020.
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