1. Αποθετήριο Παραδοτέων Υποτρόφων Δράσεων ΕΣΠΑ Τριτοβάθμιας Εκπαίδευσης 2017-2023
2. Όλο το Αποθετήριο
3. Ερευνητικές ομάδες

Τύπος:	Ανακοίνωση σε συνέδριο
Τίτλος:	Differentiation between Botryosphaeria dothidea and Neofusicoccum spp. through detection of single nucleotide polymorphisms in the ITS region
Συγγραφέας:	[EL] Παλαβούζης, Σταύρος[EN] Palavouzis, Stavros [EL] Τριανταφυλλοπούλου, Αλεξάνδρα[EN] Triantafyllopoulou, Alexandra [EL] Τζίμα, Αλίκη[EN] Tzima, Aliki [EL] Παπλωματάς, Επαμεινώντας[EN] Paplomatas, Epaminondas
Ημερομηνία:	23/08/2021
Περίληψη:	The Botryosphaeriaceae family contains a large number of fungal species causing blight, cankers and fruit rot on a plethora of plant hosts. Among the most important hosts for the members of this family is pistachio (Pistacia vera L.). For the countries surrounding the Mediterranean basin, the main species responsible for high value losses in the pistachio cultivation are Botryosphaeria dothidea, Neofusicoccum vitifusiforme, N. mediterraneum and N. parvum. Since, too many species are implicated as causal agents in symptom development, the differentiation between them is of crucial importance. To resolve that, specific primers were designed on the ITS region utilizing detection of single nucleotide polymorphisms (SNPs) due to limited sequence variation within these species. Newly designed primers were combined with generic upstream ITS primers. Initially, downstream SNP primers were designed with a single 3’ SNP base, T for B. dothidea and A for Neofusicoccum spp. However, reproducible and consistent differentiation proved difficult with these primers. As such, a different approach was implemented. Based on the initial SNP primers, new downstream SNP primers were designed. They included a mismatch next to the 3’ base and a different 5’ tail for each primer. The mismatch was different for each primer. The new approach was successful in the differentiation of the selected species. Further research regarding the design of rapid, reliable and cost efficient molecular markers for the identification between species of the Botryosphaeriaceae is underway. More specifically, Taqman primers and probes are being designed and will be evaluated on the mating type MAT1-2-1 and MAT1-1-1 gene sequences of B. dothidea, N. mediterraneum and N. parvum.
Γλώσσα:	Αγγλικά
Τόπος δημοσίευσης:	Rome, Italy
Σελίδες:	56
Θεματική κατηγορία:	[EL] Γεωπονία, διεπιστημονική προσέγγιση[EN] Agriculture, interdisciplinary
Λέξεις-κλειδιά:	Camarosporium blight; Mismatch; molecular diagnosis; SNP
Κάτοχος πνευματικών δικαιωμάτων:	© The Author(s) 2021
Ηλεκτρονική διεύθυνση του τεκμηρίου στον εκδότη:	Not available - online conference site doesn't exist anymore
Ηλεκτρονική διεύθυνση περιοδικού:	Not available - online conference site doesn't exist anymore
Σελίδες τεκμηρίου (στην πηγή):	56
Όνομα εκδήλωσης:	ICPPD018 2021: XV. International Conference on Phytopathology and Plant Disorders
Τοποθεσία εκδήλωσης:	Rome, Italy
Ημ/νία έναρξης εκδήλωσης:	23/08/2021
Ημ/νία λήξης εκδήλωσης:	24/08/2021
Σημειώσεις:	The site of the conference is not available anymore. It was an online conference during COVID with title "Congress : ICPPP031 2021: 15. International Conference on Phytopathology and Plant Pathology". The current conference site for this year is https://www.icpp2023.org/. This Research Project is co-financed by Greece and the European Union (European Social Fund) through the Operational Program “Human Resources Development, Education and Lifelong Learning 2014-2020” and the Program encoded EDBM103, titled “Support for researchers with an emphasis on young researchers-cycle B '”, in the context of the project “Risk evaluation of development of a resistant form of disease via transfer of mating type genes from the Botryosphaeria dothidea species to species of the genus Neofusicoccum” (MIS 5048471).
Εμφανίζεται στις συλλογές:	Ερευνητικές ομάδες